多组学分析揭示了杨树中香豆素类代谢物的遗传结构和局部适应性。

Multi-omics analysis reveals genetic architecture and local adaptation of coumarins metabolites in Populus.

作者信息

Zhang Wenke, Jin Zhuoying, Huang Rui, Huang Weixiong, Li Lianzheng, He Yuling, Zhou Jiaxuan, Tian Chongde, Xiao Liang, Li Peng, Quan Mingyang, Zhang Deqiang, Du Qingzhang

机构信息

State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, Beijing, 100083, P. R. China.

Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, Beijing, 100083, P. R. China.

出版信息

BMC Plant Biol. 2024 Dec 6;24(1):1170. doi: 10.1186/s12870-024-05894-9.

DOI:10.1186/s12870-024-05894-9

PMID:39643871

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11622574/

Abstract

BACKGROUND

Accumulation of coumarins plays key roles in response to immune and abiotic stress in plants, but the genetic adaptation basis of controlling coumarins in perennial woody plants remain unclear.

RESULTS

We detected 792 SNPs within 334 genes that were significantly associated with the phenotypic variations of 15 single-metabolic traits and multiple comprehensive index, such as principal components (PCs) of coumarins metabolites. Expression quantitative trait locus mapping uncovered that 337 eQTLs associated with the expression levels of 132 associated genes. Selective sweep revealed 55 candidate genes have potential selective signature among three geographical populations, highlighting that the coumarins biosynthesis have been encountered forceful local adaptation. Furthermore, we constructed a genetic network of seven candidate genes that coordinately regulate coumarins biosynthesis, revealing the multiple regulatory patterns affecting coumarins accumulation in Populus tomentosa. Validation of candidate gene variations in a drought-tolerated population and DUF538 heterologous transformation experiments verified the function of candidate genes and their roles in adapting to the different geographical conditions in poplar.

CONCLUSIONS

Our study uncovered the genetic regulation of the coumarins metabolic biosynthesis of Populus, and offered potential clues for drought-tolerance evaluation and regional improvement in woody plants.

摘要

背景

香豆素的积累在植物对免疫和非生物胁迫的响应中起关键作用，但多年生木本植物中控制香豆素的遗传适应基础仍不清楚。

结果

我们在334个基因中检测到792个单核苷酸多态性（SNP），这些基因与15个单代谢性状和多个综合指标的表型变异显著相关，如香豆素代谢物的主成分（PC）。表达数量性状基因座定位发现337个与132个相关基因表达水平相关的eQTL。选择性扫描揭示了55个候选基因在三个地理种群中具有潜在的选择特征，突出表明香豆素生物合成经历了强烈的局部适应。此外，我们构建了一个由七个候选基因组成的遗传网络，这些基因协同调节香豆素的生物合成，揭示了影响毛白杨香豆素积累的多种调控模式。在耐旱种群中对候选基因变异的验证以及DUF538异源转化实验验证了候选基因的功能及其在杨树适应不同地理条件中的作用。