Lin Chia-Yang, Lin Yu-Chun, Lu Yun-Heng, Chen Shiang-Jiuun, Lin Yu-Hsien, Tseng Yao-Kuang, Lin Yu-Tzu, Wu Yueh-Lung, Huang Rong-Nan
Department of Entomology, National Taiwan University, Taipei 106, Taiwan.
Department of Life Science, Institute of Ecology and Evolutionary Biology and TechComm-5, College of Life Science, National Taiwan University, Taipei 106, Taiwan.
Ecotoxicol Environ Saf. 2025 Jan 1;289:117430. doi: 10.1016/j.ecoenv.2024.117430. Epub 2024 Dec 6.
This study aimed to elucidate the possible synergistic effects of chemical pesticides and viral infections. Our experiments demonstrated that the Varroa mite-borne deformed wing virus (DWV) by itself had a minimal impact on bees. Conversely, when bees were simultaneously treated with acaricides, their mortality rate increased. The administration of DWV alone boosted the expression of immune response genes, whereas acaricide alone did not significantly affect the expression of detoxification genes. However, simultaneous treatment of DWV and acaricide increased both the immune response and detoxification gene expression, thereby indicating enhanced bee resistance. These findings indicate a synergistic association between viral infection and bee sensitivity to acaricides, possibly as a result of physiological or immune system impairment. Our results also indicated that adenosine supplementation enhances the resilience of bees to environmental challenges, further supporting the energy requirement hypothesis.
本研究旨在阐明化学农药与病毒感染可能存在的协同效应。我们的实验表明，由瓦螨传播的变形翅病毒（DWV）单独存在时对蜜蜂的影响极小。相反，当蜜蜂同时接受杀螨剂处理时，其死亡率会增加。单独施用DWV会促进免疫反应基因的表达，而单独使用杀螨剂对解毒基因的表达没有显著影响。然而，DWV和杀螨剂同时处理会增加免疫反应和解毒基因的表达，从而表明蜜蜂的抗性增强。这些发现表明病毒感染与蜜蜂对杀螨剂的敏感性之间存在协同关联，这可能是生理或免疫系统受损的结果。我们的结果还表明，补充腺苷可增强蜜蜂对环境挑战的恢复力，进一步支持能量需求假说。
