Liu Tongfei, Lin Lin, Pan Yun, Lin Xiaoling, Liang Ming, Shao Guanming, Feng Keyu, Liu Yaxin, Zhang Xinheng, Xie Qingmei
State Key Laboratory of Swine and Poultry Breeding Industry & Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, College of Animal Science, South China Agricultural University, Guangzhou 51064, China; Guangdong Provincial Key Lab of AgroAnimal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China; Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou 510642, China; Zhongshan Innovation Center of South China Agricultural University, Zhongshan 528400, China.
State Key Laboratory of Swine and Poultry Breeding Industry & Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, College of Animal Science, South China Agricultural University, Guangzhou 51064, China; Guangdong Provincial Key Lab of AgroAnimal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China; Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou 510642, China; Zhongshan Innovation Center of South China Agricultural University, Zhongshan 528400, China.
Poult Sci. 2025 Jan;104(1):104388. doi: 10.1016/j.psj.2024.104388. Epub 2024 Oct 18.
Infectious bursal disease (IBD), triggered by the infectious bursal disease virus (IBDV), poses a substantial risk to the poultry industry due to its immunosuppressive nature and the emergence of highly virulent strains. Traditional vaccination strategies have limitations, prompting the need for novel approaches. This study aimed to develop a recombinant Newcastle disease virus (NDV) vector vaccine co-expressing IBDV VP2 and VP3 proteins to enhance immunogenicity and protective efficacy against IBDV. The recombinant Newcastle disease virus (rNDV) expressing both VP2 and VP3 (rNDV-VP2-VP3) was generated and compared to rNDV expressing VP2 alone (rNDV-VP2). The genetic stability and growth pattern of rNDV were evaluated and its immunogenicity was assessed in specific pathogen free (SPF) chickens. rNDV-VP2-VP3 vaccines induced higher levels of neutralising antibodies, no damage to immune organs, and significantly lower viral loads in the bursa of the falciparum. rNDV-VP2 group showed partial protection, while the placebo group exhibited severe lesions and higher mortality, suggesting that the vaccine was effective in preventing IBDV-induced damage. These findings suggest that co-expression of VP2 and VP3 in NDV vectors is a viable strategy for the development of an effective IBDV vaccine, providing a safe and effective method for controlling IBD in poultry.
传染性法氏囊病（IBD）由传染性法氏囊病病毒（IBDV）引发，因其免疫抑制特性以及高致病性毒株的出现，对家禽业构成了重大风险。传统的疫苗接种策略存在局限性，因此需要新的方法。本研究旨在开发一种共表达IBDV VP2和VP3蛋白的重组新城疫病毒（NDV）载体疫苗，以增强对IBDV的免疫原性和保护效力。构建了同时表达VP2和VP3的重组新城疫病毒（rNDV-VP2-VP3），并与仅表达VP2的rNDV（rNDV-VP2）进行比较。评估了rNDV的遗传稳定性和生长模式，并在无特定病原体（SPF）鸡中评估了其免疫原性。rNDV-VP2-VP3疫苗诱导产生了更高水平的中和抗体，对免疫器官无损伤，且法氏囊中病毒载量显著更低。rNDV-VP2组表现出部分保护作用，而安慰剂组则出现严重病变且死亡率更高，这表明该疫苗在预防IBDV诱导的损伤方面是有效的。这些发现表明，在NDV载体中共表达VP2和VP3是开发有效IBDV疫苗的可行策略，为控制家禽IBD提供了一种安全有效的方法。
