• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过酶联免疫吸附测定法检测小鼠血清中抗芬太尼抗体的实验方案。

Protocol for measuring anti-fentanyl antibodies in mouse serum by enzyme-linked immunosorbent assay.

作者信息

Koster Jacob A, Prevost-Reilly Alejandra, Soni Dheeraj, Kelly Aisling, Walker Kristina, Norton Elizabeth B, Levy Ofer, Haile Colin N, Dowling David J

机构信息

Precision Vaccines Program, Department of Pediatrics, Boston Children's Hospital, Boston, MA 02115, USA.

Precision Vaccines Program, Department of Pediatrics, Boston Children's Hospital, Boston, MA 02115, USA; Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA; Global Investigative Toxicology, Preclinical Safety, Sanofi, Cambridge, MA, USA.

出版信息

STAR Protoc. 2024 Dec 20;5(4):103402. doi: 10.1016/j.xpro.2024.103402. Epub 2024 Dec 6.

DOI:10.1016/j.xpro.2024.103402
PMID:39645655
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11667700/
Abstract

Enzyme-linked immunosorbent assay (ELISA) offers an effective, inexpensive, and reliable approach for the analysis of humoral immune responses. Here, we describe a protocol for measuring anti-fentanyl antibodies generated by the immunization of mice with novel opioid vaccine candidates. We describe steps for coating BSA-fentanyl antigen and standard wells. We then detail procedures for preparing buffers and performing assays. This protocol is adaptable for use with either BALB/c or C57BL/6J mice strains and can measure antibody isotypes to elucidate class switching. For complete details on the use and execution of this protocol, please refer to Stone et al..

摘要

酶联免疫吸附测定（ELISA）为体液免疫反应分析提供了一种有效、廉价且可靠的方法。在此，我们描述了一种用于检测用新型阿片类疫苗候选物免疫小鼠后产生的抗芬太尼抗体的方案。我们描述了包被牛血清白蛋白-芬太尼抗原和标准孔的步骤。然后详细说明了制备缓冲液和进行测定的程序。该方案适用于BALB/c或C57BL/6J小鼠品系，并且可以检测抗体亚型以阐明类别转换。有关该方案的使用和实施的完整详细信息，请参考斯通等人的文献。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3e9/11667700/3ec98c7e6646/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3e9/11667700/dd1bcb3c93e9/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3e9/11667700/7b491a7e3011/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3e9/11667700/261673acca93/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3e9/11667700/3414e076865a/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3e9/11667700/b1470e52b2fd/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3e9/11667700/d279e5e00d80/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3e9/11667700/b9b916f7d7d8/gr11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3e9/11667700/988a218952b5/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3e9/11667700/7c956d44f5db/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3e9/11667700/6cf4ce2e168f/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3e9/11667700/56aae1c2b364/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3e9/11667700/3ec98c7e6646/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3e9/11667700/dd1bcb3c93e9/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3e9/11667700/7b491a7e3011/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3e9/11667700/261673acca93/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3e9/11667700/3414e076865a/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3e9/11667700/b1470e52b2fd/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3e9/11667700/d279e5e00d80/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3e9/11667700/b9b916f7d7d8/gr11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3e9/11667700/988a218952b5/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3e9/11667700/7c956d44f5db/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3e9/11667700/6cf4ce2e168f/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3e9/11667700/56aae1c2b364/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3e9/11667700/3ec98c7e6646/gr7.jpg

相似文献

1
Protocol for measuring anti-fentanyl antibodies in mouse serum by enzyme-linked immunosorbent assay.通过酶联免疫吸附测定法检测小鼠血清中抗芬太尼抗体的实验方案。
STAR Protoc. 2024 Dec 20;5(4):103402. doi: 10.1016/j.xpro.2024.103402. Epub 2024 Dec 6.
2
Protocol to measure the impact of Clostridioides difficile toxins on antibody responses using ELISA, ELISPOT, and toxin-neutralization assays.使用酶联免疫吸附测定（ELISA）、酶联免疫斑点测定（ELISPOT）和毒素中和试验来测量艰难梭菌毒素对抗体反应影响的方案。
STAR Protoc. 2025 Jun 20;6(2):103754. doi: 10.1016/j.xpro.2025.103754. Epub 2025 Apr 8.
3
A novel cationic liposome-formulated toll like receptor (TLR) 7/8 agonist enhances the efficacy of a vaccine against fentanyl toxicity.一种新型阳离子脂质体配方的Toll样受体（TLR）7/8激动剂可增强抗芬太尼毒性疫苗的效力。
J Control Release. 2025 Aug 10;384:113901. doi: 10.1016/j.jconrel.2025.113901. Epub 2025 May 27.
4
Antibody tests for identification of current and past infection with SARS-CoV-2.抗体检测用于鉴定 SARS-CoV-2 的现症感染和既往感染。
Cochrane Database Syst Rev. 2022 Nov 17;11(11):CD013652. doi: 10.1002/14651858.CD013652.pub2.
5
Opioids for the management of breakthrough pain in cancer patients.用于治疗癌症患者爆发性疼痛的阿片类药物。
Cochrane Database Syst Rev. 2013 Oct 21(10):CD004311. doi: 10.1002/14651858.CD004311.pub3.
6
Induction of antigen-specific CTL and antibody responses in mice by a novel recombinant tandem repeat DNA vaccine targeting at mucin 1 of pancreatic cancer.新型靶向胰腺癌黏蛋白 1 的串联重复 DNA 疫苗诱导小鼠产生抗原特异性 CTL 和抗体应答。
J Cancer Res Clin Oncol. 2010 Dec;136(12):1861-8. doi: 10.1007/s00432-010-0845-4. Epub 2010 Mar 13.
7
Evaluation of Anti-SARS-CoV-2 IgG Responses in a Clinical Study of a Biosimilar Candidate to Denosumab Using Singlicate Analysis.使用单份分析在一种地诺单抗生物类似药候选产品的临床研究中评估抗SARS-CoV-2 IgG反应。
Drugs R D. 2025 May 23. doi: 10.1007/s40268-025-00510-z.
8
Systemic pharmacological treatments for chronic plaque psoriasis: a network meta-analysis.系统性药理学治疗慢性斑块状银屑病：网络荟萃分析。
Cochrane Database Syst Rev. 2021 Apr 19;4(4):CD011535. doi: 10.1002/14651858.CD011535.pub4.
9
Divergent ventilatory responses during opioid-induced respiratory depression in response to repeated fentanyl use.重复使用芬太尼时，阿片类药物诱发呼吸抑制过程中不同的通气反应。
Am J Physiol Lung Cell Mol Physiol. 2025 Jul 1;329(1):L161-L171. doi: 10.1152/ajplung.00302.2024. Epub 2025 Jun 2.
10
Capillary driven microfluidic sequential flow device for point-of-need ELISA: COVID-19 serology testing.用于即时检测 ELISA 的毛细驱动微流控顺序流动装置：COVID-19 血清学检测。
Anal Methods. 2023 Jun 8;15(22):2721-2728. doi: 10.1039/d3ay00225j.

本文引用的文献

1
Fentanyl conjugate vaccine by injected or mucosal delivery with dmLT or LTA1 adjuvants implicates IgA in protection from drug challenge.通过注射或黏膜给药方式接种含有dmLT或LTA1佐剂的芬太尼缀合物疫苗，可使IgA参与抵御药物攻击的保护作用。
NPJ Vaccines. 2021 May 13;6(1):69. doi: 10.1038/s41541-021-00329-0.