Kloc Anna, Wójcik-Fatla Angelina, Paprzycki Piotr, Panasiuk Lech
Department of Health Biohazards and Parasitology, Institute of Rural Health, Lublin, Poland.
Department of Medical Diagnostic Research, Institute of Rural Health, Lublin, Poland.
Ticks Tick Borne Dis. 2024 Nov;15(6):102421. doi: 10.1016/j.ttbdis.2024.102421. Epub 2024 Dec 8.
Research on the transovarial transmission of pathogens whose reservoirs and vectors are ticks has led to an understanding of the mechanisms related to the circulation and persistence of selected microorganisms in natural foci. The primary aim of this study was to investigate the possibility of transovarial transmission of Rickettsia spp. in Dermacentor reticulatus ticks, and the influence of Francisella-like endosymbionts (FLEs) and Spiroplasma spp. on the efficiency of the egg-laying process and transmission of selected pathogens. In total, 16,600 eggs were obtained under laboratory conditions from 55 females, with an average of 346 eggs per female. Adults, eggs, and hatched larvae were tested using polymerase chain reaction (PCR) for the presence of Rickettsia and endosymbionts. DNA fragments of Rickettsia spp. were found in females (56.4 %) and in pools of eggs (72.9 %) and larvae (62.4 %). FLEs and Spiroplasma endosymbionts were confirmed in females (80 % and 14.5 %, respectively), pools of eggs (81.6 % and 26.1 %, respectively), and larvae (82.7 % and 46.2 %, respectively). Transovarial transmission was confirmed in Rickettsia raoultii, FLEs, and Spiroplasma ixodetis. No correlation was observed between the occurrence of individual endosymbionts and the efficiency of egg laying and transovarial transmission in Rickettsia spp. In conclusion, transovarial transmission of Rickettsia spp., FLEs and Spiroplasma spp. in D. reticulatus plays an important role in their persistence and circulation in the environment. However, further research is required on this topic.
对病原体经卵传递的研究，这些病原体的储存宿主和传播媒介为蜱，这使人们对某些微生物在自然疫源地中的循环和持续存在相关机制有了一定了解。本研究的主要目的是调查立克次氏体属在网纹革蜱中的经卵传递可能性，以及类弗朗西斯菌内共生体（FLEs）和螺原体属对产卵过程效率和所选病原体传播的影响。在实验室条件下，共从55只雌蜱获得了16,600枚卵，平均每只雌蜱346枚卵。使用聚合酶链反应（PCR）检测成虫、卵和孵化出的幼虫中是否存在立克次氏体和内共生体。在雌蜱（56.4%）、卵池（72.9%）和幼虫（62.4%）中发现了立克次氏体属的DNA片段。在雌蜱（分别为80%和14.5%）、卵池（分别为81.6%和26.1%）和幼虫（分别为82.7%和46.2%）中证实存在FLEs和螺原体属内共生体。在拉乌尔立克次氏体、FLEs和嗜蜱螺原体中证实了经卵传递。在立克次氏体属中，未观察到单个内共生体的存在与产卵效率和经卵传递之间的相关性。总之，立克次氏体属、FLEs和螺原体属在网纹革蜱中的经卵传递在它们于环境中的持续存在和循环中起着重要作用。然而，关于这个主题还需要进一步研究。
