Shilova Nadezhda V, Polyakova Svetlana M, Nokel Alexey Yu, Lipatnikov Alexander D, Gordeeva Elena A, Lavrenteva Marina V, Bovin Nicolai V
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia.
National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V. I. Kulakov of the Ministry of Health of the Russian Federation, Moscow, 117997, Russia.
Biochemistry (Mosc). 2024 Nov;89(11):2023-2027. doi: 10.1134/S0006297924110142.
Fluorescently labeled and conjugated (strept)avidins are widely used for imaging biotinylated molecules in immunological assays and histochemistry. We showed that besides biotin, these proteins bind glycans, including fragments of mammalian glycoproteins and glycolipids, in particular, ABO blood group antigens, oligolactosamines, and 6--sulfated oligosaccharides. This interaction is inhibited in a dose-dependent manner by micromolar concentrations of polymeric, but not monomeric, glycan conjugates (i.e., requires polyvalence). Taking into account the cluster organization of cell glycans (glycoproteins and glycolipids), the ability of (strept)avidins to bind glycans might be a source of errors in the analysis of carbohydrate-containing samples, which can be prevented by avoiding a large excess of (strept)avidin in analytical systems.
荧光标记和偶联的（链）抗生物素蛋白广泛用于免疫分析和组织化学中对生物素化分子的成像。我们发现，除了生物素外，这些蛋白质还能结合聚糖，包括哺乳动物糖蛋白和糖脂的片段，特别是ABO血型抗原、低聚乳糖胺和6-硫酸化寡糖。这种相互作用受到微摩尔浓度的聚合聚糖偶联物（而非单体聚糖偶联物）的剂量依赖性抑制（即需要多价性）。考虑到细胞聚糖（糖蛋白和糖脂）的簇状组织，（链）抗生物素蛋白结合聚糖的能力可能是含碳水化合物样品分析中误差的来源，通过在分析系统中避免大量过量的（链）抗生物素蛋白可以防止这种误差。
