Kim Soyeon, Larnani Sri, Taymour Noha, Chung Shin Hye, Srinivasan Murali, Kim Young-Jae, Park Young-Seok
Department of Oral Anatomy and Dental Research Institute, School of Dentistry, Seoul National University.
Department of Substitutive Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University.
J Oral Sci. 2025 Jan 16;67(1):14-18. doi: 10.2334/josnusd.24-0287. Epub 2024 Dec 6.
Coffee consumption is a well-known contributor to tooth discoloration, and the extent of staining is influenced by the chemical composition of the coffee. This study investigated the associations of coffee roasting level, chlorogenic acid (CGA) content, absorbance level, and their combined effects with tooth discoloration.
Bovine tooth enamel specimens were immersed in light, medium, and dark roasts of four coffee types (two Arabica and two Robusta coffees) for 72 h. High-performance liquid chromatography (HPLC) was used to measure CGA content, absorbance levels were estimated by using pigment concentration, and discoloration was assessed by spectrophotometry. The data were analyzed with the Friedman test.
Medium roasts induced the greatest discoloration, and tooth specimens immersed in Ethiopia Arabica exhibited the greatest color difference based on CIEDE2000 (ΔE at 72 h: 13.51 ± 4.63). Light roasts induced the least staining, despite having the highest CGA content. Dark roasts showed the highest absorbance, indicating a higher pigment concentration. Friedman analysis revealed a significant difference in color change in relation to roasting level for all coffee types.
The present findings indicate that tooth discoloration is caused by the complex interaction of CGA, melanoidins, and roasting level. Because of the interplay of these factors, medium roasting had the greatest effect on discoloration.
咖啡饮用是牙齿变色的一个众所周知的因素,染色程度受咖啡化学成分的影响。本研究调查了咖啡烘焙程度、绿原酸(CGA)含量、吸光度水平及其综合作用与牙齿变色之间的关联。
将牛牙釉质标本浸泡在四种咖啡(两种阿拉比卡咖啡和两种罗布斯塔咖啡)的浅度、中度和深度烘焙咖啡中72小时。使用高效液相色谱法(HPLC)测量CGA含量,通过色素浓度估算吸光度水平,并通过分光光度法评估变色情况。数据采用弗里德曼检验进行分析。
中度烘焙引起的变色最大,基于CIEDE2000,浸泡在埃塞俄比亚阿拉比卡咖啡中的牙齿标本颜色差异最大(72小时时的ΔE:13.51±4.63)。浅度烘焙引起的染色最少,尽管其CGA含量最高。深度烘焙显示出最高的吸光度,表明色素浓度较高。弗里德曼分析显示,所有咖啡类型的颜色变化在烘焙程度方面存在显著差异。
目前的研究结果表明,牙齿变色是由CGA、类黑素和烘焙程度的复杂相互作用引起的。由于这些因素的相互作用,中度烘焙对变色的影响最大。