Narita Takumi, Hsieh Wen-Chuan, Ku Yu Tzu, Su Yu-Chieh, Inoguchi Hiroki, Takeno Hiroyuki
Division of Molecular Science, Graduate School of Science and Technology, Gunma University, 1-5-1 Tenjin, Kiryu 376-8515, Gunma, Japan.
Department of Medical Science and Biotechnology, College of Medicine, I-SHOU University, No.8, Yida, Kaohsiung 824005, Taiwan, ROC.
Biomacromolecules. 2025 Jan 13;26(1):374-386. doi: 10.1021/acs.biomac.4c01199. Epub 2024 Dec 8.
We investigated the fracture behavior of cellulose nanofiber (CNF)-reinforced poly(vinyl alcohol) (PVA) hydrogels cross-linked with borax and the effect of freeze-thaw (FT) cycles on it. The CNF/PVA/Borax hydrogel not subjected to FT achieved a fracture energy of 5.8 kJ m and a dissipative length of 2.3 mm, comparable to those of tough hydrogels. Lacking either CNF or borax remarkably decreased the fracture energy and the dissipative length; CNF contributed to a physical blocking of the crack growth, whereas the complexations between CNF and borate yielded nonlocalization of energy dissipation. Repeated FT cycles markedly improved the mechanical performance of unnotched samples, but they decreased the fracture energy due to the lowering of the dissipative length. Besides, CNF/PVA/Borax hydrogels were suitable for cell scaffold materials. The culture of umbilical cord mesenchymal stem cells (UC-MSCs) revealed a positive correlation between culture duration and the number of UC- MSCs adherent to the material.
我们研究了与硼砂交联的纤维素纳米纤维（CNF）增强聚乙烯醇（PVA）水凝胶的断裂行为以及冻融（FT）循环对其的影响。未经过FT处理的CNF/PVA/硼砂水凝胶的断裂能达到5.8 kJ/m²，耗散长度为2.3 mm，与坚韧水凝胶相当。缺少CNF或硼砂会显著降低断裂能和耗散长度；CNF有助于物理阻止裂纹扩展，而CNF与硼酸盐之间的络合作用产生了能量耗散的非局部化。重复的FT循环显著提高了无缺口样品的机械性能，但由于耗散长度降低，它们降低了断裂能。此外，CNF/PVA/硼砂水凝胶适用于细胞支架材料。脐带间充质干细胞（UC-MSCs）的培养显示培养持续时间与粘附在该材料上的UC-MSCs数量之间呈正相关。
