Dinsa Guta Rahel, Semunyana Marc, Arif Saima, Jeong Inyong, Kim Sun Ha, Min Jiyoung, Oh Sang-Keun
Department of Applied Biology, Chungnam National University, Daejeon, South Korea.
Mycobiology. 2024 Nov 17;52(5):306-316. doi: 10.1080/12298093.2024.2408064. eCollection 2024.
is a significant phytopathogen causing downy mildew disease in cucurbit crops. Understanding the molecular mechanisms underlying the interaction between and its host is essential for developing effective disease management strategies. RxLR effectors, secreted by pathogens, play a crucial role in modulating host immunity. In this study, we sequenced the genome of the isolate CNU-OTH and identified RxLR effector genes using bioinformatics tools. A total of 45 RxLR effector genes were identified from the genome of . Cloning and functional characterization of these effectors were performed through transient expression assays in leaves. Subcellular localization of selected effectors was determined using GFP-tagged constructs. Functional characterization revealed that while most effectors did not induce a hypersensitive response (HR), a subset showed either weak or strong necrosis. Furthermore, several effectors demonstrated the ability to suppress cell death induced by BAX and INF1. Subcellular localization analysis indicated that RxLR effectors exhibited fluorescence in the nucleus and plasma membrane of cells, suggesting diverse roles in host-pathogen interactions. This study provides insights into the genetic diversity and functional characterization of RxLR effectors in . Understanding the role of these effectors in manipulating host immunity is critical for developing strategies to combat downy mildew disease in cucurbit crops. The findings contribute to the broader understanding of plant-pathogen interactions and may facilitate the development of disease-resistant crop varieties.
是一种导致葫芦科作物霜霉病的重要植物病原体。了解其与宿主之间相互作用的分子机制对于制定有效的病害管理策略至关重要。病原体分泌的RxLR效应子在调节宿主免疫中起关键作用。在本研究中，我们对分离株CNU - OTH的基因组进行了测序，并使用生物信息学工具鉴定了RxLR效应子基因。从的基因组中总共鉴定出45个RxLR效应子基因。通过在叶片中的瞬时表达分析对这些效应子进行了克隆和功能表征。使用带有GFP标签的构建体确定了所选效应子的亚细胞定位。功能表征表明，虽然大多数效应子未诱导超敏反应（HR），但一部分显示出弱或强坏死。此外，几个效应子表现出抑制由BAX和INF1诱导的细胞死亡的能力。亚细胞定位分析表明，RxLR效应子在细胞的细胞核和质膜中呈现荧光，表明其在宿主 - 病原体相互作用中具有多种作用。本研究为中RxLR效应子的遗传多样性和功能表征提供了见解。了解这些效应子在操纵宿主免疫中的作用对于制定防治葫芦科作物霜霉病的策略至关重要。这些发现有助于更广泛地理解植物 - 病原体相互作用，并可能促进抗病作物品种的开发。
