Yeni Yesim, Nadaroglu Hayrunnisa, Ertugrul M Sait, Hacimuftuoglu Ahmet, Alayli Azize
Department of Medical Pharmacology, Faculty of Medicine, Malatya Turgut Ozal University, Malatya 44280, Turkey.
Department of Food Technology, Vocational College of Technical Sciences, Ataturk University, Erzurum 25240, Turkey.
Toxicol Rep. 2024 Nov 19;13:101818. doi: 10.1016/j.toxrep.2024.101818. eCollection 2024 Dec.
Nanoparticles are attracting attention for their potential therapeutic applications, particularly in cancer therapy, underscoring their importance in medicine. Cadmium sulfide nanoparticles, known for their robust catalytic and optical properties, are classified as chalcogenides and show promise for cancer diagnosis and treatment. Neuroblastoma, a common solid tumor in childhood, poses a significant health threat with different outcomes depending on its biological subtype. This study evaluated the antiproliferative effects of cadmium sulfide nanoparticles on the SY-SH5Y cell line. Walnut shell extract and NaS were used to facilitate the synthesis of cadmium sulfide nanoparticles by green synthesis. Characterization of the synthesized cadmium sulfide nanoparticles was performed by Fourier transform infrared spectroscopy, scanning electron microscopy, and x-ray diffraction analyses. The SH-SY5Y cell line was cultured in a standard cell culture medium and then exposed to different cadmium sulfide nanoparticles (10-25-50-75-100 µg/mL) for 24 hours. Cell viability, oxidant, and antioxidant levels were then assessed using a 3-(4,5-dimetiltiyazol-2-il)-2,5-difeniltetrazolyum bromür, total antioxidant, and total oxidant assays. The data showed that applying 100 μg/mL cadmium sulfide nanoparticles resulted in a significant decrease in cancer cell viability of up to 40.96 % (<0.05). The cadmium sulfide nanoparticles had a dose-dependent effect on the SH-SY5Y cell line. Furthermore, cadmium sulfide nanoparticles increased oxidative activity in neuroblastoma cells, which was consistent with the results of the 3-(4,5-dimetiltiyazol-2-il)-2,5-difeniltetrazolyum bromür assay. In conclusion, cadmium sulfide nanoparticles exhibited potent activity against the neuroblastoma cell. This study highlights the antiproliferative efficacy of green-synthesized cadmium sulfide nanoparticles with walnut shell extract on relevant cancer cell lines.
纳米颗粒因其潜在的治疗应用而备受关注，尤其是在癌症治疗方面，这凸显了它们在医学中的重要性。硫化镉纳米颗粒以其强大的催化和光学特性而闻名，属于硫属化物，在癌症诊断和治疗方面显示出前景。神经母细胞瘤是儿童常见的实体瘤，根据其生物学亚型不同，会对健康构成重大威胁。本研究评估了硫化镉纳米颗粒对SY-SH5Y细胞系的抗增殖作用。利用核桃壳提取物和硫化钠通过绿色合成法来促进硫化镉纳米颗粒的合成。通过傅里叶变换红外光谱、扫描电子显微镜和X射线衍射分析对合成的硫化镉纳米颗粒进行表征。将SH-SY5Y细胞系培养在标准细胞培养基中，然后暴露于不同浓度（10-25-50-75-100μg/mL）的硫化镉纳米颗粒中24小时。随后使用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐、总抗氧化剂和总氧化剂检测法评估细胞活力、氧化剂和抗氧化剂水平。数据显示，施加100μg/mL的硫化镉纳米颗粒可使癌细胞活力显著降低高达40.96%（<0.05）。硫化镉纳米颗粒对SH-SY5Y细胞系具有剂量依赖性作用。此外，硫化镉纳米颗粒增加了神经母细胞瘤细胞中的氧化活性，这与3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐检测结果一致。总之，硫化镉纳米颗粒对神经母细胞瘤细胞表现出强大的活性。本研究突出了用核桃壳提取物绿色合成的硫化镉纳米颗粒对相关癌细胞系的抗增殖功效。
