Mei Jiaqi, Tang Xiaoyu, Gu Yujie, Lu Huijie, Yang Ying, Shen Qinyuan, Yang Lingwei, Li Bei, Zuo Jianfang, Singh Vijay Pratap, Sharma Anket, Yuan Huwei, Zheng Bingsong
State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou, China.
Plant Physiology Laboratory, Department of Botany, Chaudhary Mahadeo Prasad (C.M.P.) Degree College, University of Allahabad, Prayagraj, India.
Front Plant Sci. 2024 Nov 22;15:1494579. doi: 10.3389/fpls.2024.1494579. eCollection 2024.
Auxins play significant roles in plant growth and development. The transporter inhibitor response1/auxin signaling F-box () gene family encodes the auxin receptor proteins and plays an essential role in the auxin signaling pathway. Here we identified and characterized the family in (Cc) plants (named as ). Seven were identified and further confirmed by cloning. All proteins encoded by these genes conservatively contained two domains, the F-box and leucine-rich repeat (LRR) domains. The were located in the nucleus. Phylogenetic analysis suggested that CcTIR1/AFBs were evenly scattered in four different subgroups. The cis-acting element analysis indicates that might be activated by auxin. The spatial and temporal expression of during grafting suggested that both and in scions and in the rootstocks were significantly upregulated at 3 days after grafting, which indicated the specialization of three during grafting. The Y2H assay indicated that three CcAFBs were capable of interacting with CcIAA16, CcIAA27b, and CcIAA29a, among which CcAFB4 interacted strongly with CcIAA1 and CcIAA16. Our study provides the opportunity to understand the potential role of not only but also special (, , and ), which is a great aspect to further explore the molecular mechanism during the grafting process.
生长素在植物生长发育中发挥着重要作用。转运蛋白抑制响应1/生长素信号F-box（TIR1/AFB）基因家族编码生长素受体蛋白，在生长素信号通路中起关键作用。在此，我们在山茶属（Camellia）植物中鉴定并表征了TIR1/AFB家族（命名为CcTIR1/AFBs）。通过克隆鉴定出7个CcTIR1/AFBs，并进一步得到证实。这些基因编码的所有蛋白质均保守地包含两个结构域，即F-box和富含亮氨酸重复序列（LRR）结构域。CcTIR1/AFBs定位于细胞核。系统发育分析表明，CcTIR1/AFBs均匀分布在四个不同的亚组中。顺式作用元件分析表明，CcTIR1/AFBs可能受生长素激活。嫁接过程中CcTIR1/AFBs的时空表达表明，接穗中的CcTIR1、CcAFB4和CcAFB5以及砧木中的CcTIR1在嫁接后3天均显著上调，这表明嫁接过程中3个CcTIR1/AFBs具有特异性。酵母双杂交试验表明，3个CcAFBs能够与CcIAA16、CcIAA27b和CcIAA29a相互作用，其中CcAFB4与CcIAA1和CcIAA16强烈相互作用。我们的研究为了解CcTIR1/AFBs以及特殊的CcAFBs（CcAFB4、CcAFB5和CcTIR1）的潜在作用提供了契机，这是进一步探索嫁接过程分子机制的一个重要方面。
