Dehdari Ebrahimi Niloofar, Sadeghi Alireza, Falamarzi Kimia, Shahlaee Mohammad Amin, Azarpira Negar
Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Ann Med Surg (Lond). 2024 Oct 16;86(12):7062-7071. doi: 10.1097/MS9.0000000000002620. eCollection 2024 Dec.
Radiation exposure is a concern in today's world, given the widespread use of electronic devices and medical procedures involving ionizing and non-ionizing radiation. Radiations may cause male infertility by inducing oxidative stress in testicular tissue. Melatonin has antioxidant properties.
The authors systematically reviewed the literature for the studies that have investigated the effects of melatonin therapy on radiation-induced oxidative stress in rodents' testicular tissue. PubMed, Scopus, and Web of Science were searched for relevant animal trials. Standardized mean difference and 95% CIs were used to pool the data. Subgroup and sensitivity analyses were done. The risk of bias was assessed using SYRCLE tool.
Outcomes: histopathology and sperm analyses (testicular apoptotic cells, Johnsen's testicular biopsy score, seminiferous epithelial height, tubular diameter, sperm motility, viability, count, and morphology, concentration of spermatid, spermatocyte, and spermatogonia), body and testes weights (absolute and relative body and testicular weights), reproductive hormones (serum prolactin, FSH, and testosterone), and oxidative stress tissue markers (TBARS, CAT, GSH, GSH-Px, MDA, SOD, and XO, and total antioxidant capacity). Rats and mice were exposed to electromagnetic radiations (gamma, roentgen, microwave, radiofrequency, and high-power line energy) and particle waves (radioiodine and carbon-ion). Melatonin therapy was significantly associated with improved male reproduction.
Radiation exposure harms male fertility, but melatonin, as an antioxidant, is potentially associated with improved male reproductive function in rodents. Inconsistencies in research require further investigations.
鉴于电子设备的广泛使用以及涉及电离和非电离辐射的医疗程序，辐射暴露在当今世界是一个令人担忧的问题。辐射可能通过在睾丸组织中诱导氧化应激而导致男性不育。褪黑素具有抗氧化特性。
作者系统地回顾了文献，以查找研究褪黑素疗法对啮齿动物睾丸组织中辐射诱导的氧化应激影响的研究。在PubMed、Scopus和科学网中搜索相关的动物试验。使用标准化均数差和95%可信区间汇总数据。进行了亚组分析和敏感性分析。使用SYRCLE工具评估偏倚风险。
结果：组织病理学和精子分析（睾丸凋亡细胞、约翰森睾丸活检评分、生精上皮高度、管径、精子活力、存活率、计数和形态、精子细胞、精母细胞和精原细胞浓度）、体重和睾丸重量（绝对和相对体重及睾丸重量）、生殖激素（血清催乳素、促卵泡激素和睾酮）以及氧化应激组织标志物（硫代巴比妥酸反应物、过氧化氢酶、谷胱甘肽、谷胱甘肽过氧化物酶、丙二醛、超氧化物歧化酶和黄嘌呤氧化酶以及总抗氧化能力）。大鼠和小鼠暴露于电磁辐射（γ射线、伦琴射线、微波、射频和高压线路能量）和粒子波（放射性碘和碳离子）。褪黑素疗法与男性生殖功能改善显著相关。
辐射暴露会损害男性生育能力，但褪黑素作为一种抗氧化剂，可能与改善啮齿动物的男性生殖功能有关。研究中的不一致之处需要进一步调查。
