Mamedov T, Zakiyeva G, Demirel F, Mammadova G, Hasanova G
Department of Agricultural Biotechnology, Akdeniz University, 07058 Antalya, Turkey.
Institute of Molecular Biology and Biotechnologies, Ministry of Science and Education, Republic of Azerbaijan, AZ 1073 Baku, Azerbaijan.
Photosynthetica. 2024 Feb 5;62(1):90-101. doi: 10.32615/ps.2024.002. eCollection 2024.
Phosphoglycolate phosphatase (PGPase), a key enzyme in photosynthetic organisms, catalyzes the dephosphorylation of phosphoglycolate, which is largely produced by the oxygenase activity of Rubisco, and is a potent inhibitor of several Calvin cycle enzymes. PGPase (CrPGPase 1) was previously cloned, purified, and characterized from unicellular green . analysis revealed two more candidates encoding PGPase enzymes in the genome. In this study, we isolated, cloned, and overexpressed three PGPase genes (, , ) from and performed gene expression analysis at high and low ammonium [NH ] concentrations. We demonstrate that all three genes encode functionally active PGPases in . In addition, we show that and genes are N-responsive genes and are upregulated under low ammonium concentrations. analysis revealed that PGPase exists mainly in three isoforms in higher plants and algae.
磷酸乙醇酸磷酸酶（PGPase）是光合生物中的一种关键酶，催化磷酸乙醇酸的去磷酸化反应，磷酸乙醇酸主要由核酮糖-1,5-二磷酸羧化酶/加氧酶（Rubisco）的加氧酶活性产生，并且是几种卡尔文循环酶的有效抑制剂。PGPase（CrPGPase 1）先前已从单细胞绿藻中克隆、纯化并进行了表征。分析显示在该基因组中还有另外两个编码PGPase酶的候选基因。在本研究中，我们从[具体物种名称未给出]中分离、克隆并过表达了三个PGPase基因（[具体基因名称未给出]），并在高铵和低铵[NH₄⁺]浓度下进行了基因表达分析。我们证明所有三个[具体物种名称未给出]基因在[具体物种名称未给出]中都编码功能活性的PGPase。此外，我们表明[具体基因名称未给出]和[具体基因名称未给出]基因是氮响应基因，在低铵浓度下上调。分析显示PGPase在高等植物和藻类中主要以三种同工型存在。
