Reif A E
Cancer Res. 1985 Jan;45(1):25-31.
Adult or newborn C57BL/6J mice were immunized with isogenic Moloney strain MuLV-induced leukemia cells irradiated with 10,000 rads or treated with low concentrations of formalin. Groups of immunized and control mice were challenged with a range of doses of viable leukemia cells, and tumor deaths were recorded for 90 days after challenge. Then, the doses of challenge cells which produced 50% tumor deaths were calculated for immunized and control mice. The logarithm of their ratio quantified the degree of protection provided by immunization. For adult C57BL/6J mice, a single immunization with MuLV-induced leukemia cells was not effective; either cells plus Bacillus Calmette-Guérin or Corynebacterium parvum, or else two immunizations with irradiated leukemia cells were needed to produce statistically significant increases in the values of the doses of challenge cells which produced 50% tumor deaths. Cross-protection was obtained by immunization with other isogenic MuLV-induced leukemias, but not by immunization with isogenic carcinogen-induced tumors or with an isogenic spontaneous leukemia. For newborn mice, a single injection of irradiated leukemia cells provided 1.3 to 1.5 logs of protection, and admixture of B. Calmette-Guérin or C. parvum increased this protection to 2.4 to 2.7 logs. Since irradiated and frozen-thawed MuLV-induced leukemia cells contained viable MuLV, leukemia cells treated with 0.5 or 1.0% formalin were tested as an alternative. A single injection of formalin-treated isogenic leukemia cells admixed with C. parvum provided between 1.7 and 2.8 logs of protection. These results demonstrate that a single vaccination of newborn animals against a highly antigenic virally induced leukemia produces strong protection against a subsequent challenge with viable leukemia cells.
成年或新生的C57BL/6J小鼠用经10000拉德照射或用低浓度福尔马林处理的同基因莫洛尼株鼠白血病病毒（MuLV）诱导的白血病细胞进行免疫。将免疫组和对照组小鼠用一系列剂量的活白血病细胞进行攻击，并记录攻击后90天的肿瘤死亡情况。然后，计算免疫组和对照组小鼠产生50%肿瘤死亡的攻击细胞剂量。它们比值的对数量化了免疫提供的保护程度。对于成年C57BL/6J小鼠，用MuLV诱导的白血病细胞进行单次免疫无效；需要细胞加卡介苗或短小棒状杆菌，或者用照射过的白血病细胞进行两次免疫，才能使产生50%肿瘤死亡的攻击细胞剂量值有统计学意义的增加。用其他同基因MuLV诱导的白血病进行免疫可获得交叉保护，但用同基因致癌物诱导的肿瘤或同基因自发性白血病进行免疫则不能获得交叉保护。对于新生小鼠，单次注射照射过的白血病细胞可提供1.3至1.5对数的保护，卡介苗或短小棒状杆菌的混合使用可将这种保护提高到2.4至2.7对数。由于经照射和冻融的MuLV诱导的白血病细胞含有活的MuLV，因此测试了用0.5%或1.0%福尔马林处理的白血病细胞作为替代方法。单次注射与短小棒状杆菌混合的经福尔马林处理的同基因白血病细胞可提供1.7至2.8对数的保护。这些结果表明，对新生动物进行单次针对高度抗原性病毒诱导的白血病的疫苗接种可对随后用活白血病细胞进行的攻击产生强大的保护作用。
