Cui Guanglin, Yuan Aping, Sørbye Sveinung W, Florholmen Jon
Research Group of Gastrointestinal Diseases, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Faculty of Health Science, Nord University, Campus Levanger, Norway.
Clin Med Insights Oncol. 2024 Dec 7;18:11795549241301358. doi: 10.1177/11795549241301358. eCollection 2024.
Inflammation is the most important deriving force for the development of colitis-associated colorectal cancer (CAC) through the Inflammation-Pretumor dysplasia-CAC sequence. T helper (Th) subsets Th9 and Th17 cells can potentially stimulate inflammation in the ulcerative colitis (UC). Therefore, Th9 and Th17 cells may play a promoting role in the colitis-associated dysplasia (CAD).
Using immunohistochemistry (IHC), we evaluated the presentation patterns and densities of T lymphocytes, Th9 and Th17 cells in human UC and CAD tissues.
A general increasing trend of CD3-positive T lymphocytes, P.U.1-positive Th9 and interleukin (IL)-17A-positive Th17 cells was illustrated throughout the normal-UC-CAD sequence, IHC images showed that these cells were very prominent in the lamina propria, and some cells were also observed in the epithelium in the CAD tissues. Density analysis revealed that numbers of Th9 and Th17 cells were progressively increased in the CAD tissues as compared with the UC and control tissues. In general, densities of Th9 and Th17 cells in the lamina propria were slightly higher in the non-adenoma-like dysplasia (NALD) tissues than that in the adenoma-like dysplasia (ALD) tissues. However, densities of neither Th9 nor Th17 cells in both the ALD and NALD subgroups were associated with the degree of dysplasia in CAD lesions.
Accumulated Th9 and Th17 cells contribute to the immune cellular composition in the CAD tissues and may represent the early conditional change for the Dysplasia-CAC transition.
炎症是通过炎症-肿瘤前发育异常-结肠炎相关结直肠癌（CAC）序列促进结肠炎相关结直肠癌（CAC）发生发展的最重要驱动力。辅助性T（Th）细胞亚群Th9和Th17细胞可能会促进溃疡性结肠炎（UC）中的炎症反应。因此，Th9和Th17细胞可能在结肠炎相关发育异常（CAD）中发挥促进作用。
我们采用免疫组织化学（IHC）方法，评估了人UC和CAD组织中T淋巴细胞、Th9和Th17细胞的表达模式及密度。
在正常-UC-CAD序列中，CD3阳性T淋巴细胞、PU.1阳性Th9和白细胞介素（IL）-17A阳性Th17细胞总体呈增加趋势，免疫组化图像显示这些细胞在固有层中非常突出，在CAD组织的上皮中也观察到一些细胞。密度分析显示，与UC和对照组织相比，CAD组织中Th9和Th17细胞数量逐渐增加。一般来说，非腺瘤样发育异常（NALD）组织固有层中Th9和Th17细胞的密度略高于腺瘤样发育异常（ALD）组织。然而，ALD和NALD亚组中Th9和Th17细胞的密度均与CAD病变的发育异常程度无关。
Th9和Th17细胞的积累有助于CAD组织中的免疫细胞组成，可能代表发育异常向CAC转变的早期条件性变化。
