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尿地高辛放射免疫测定法中的陷阱及新方法

Pitfalls in, and new methods for, the radioimmunoassay for digoxin in urine.

作者信息

O'Brien M S, Huston J R, Reid R J, Gibson T P

出版信息

Clin Chem. 1985 Jan;31(1):122-4.

PMID:3965187
Abstract

Examining techniques for radioimmunoassay of digoxin in urine, we found that the apparent concentration of digoxin is affected by endogenous substances, independent of the concentration of digoxin itself. We describe a modification of the Becton Dickinson solid-phase RIA that makes it more nearly accurate and more easily performed, and obviates the endogenous interference. Digoxin concentrations from 5.0 to at least 1000 micrograms/L can be measured.

摘要

在研究尿中地高辛放射免疫测定的检测技术时,我们发现地高辛的表观浓度受内源性物质影响,与地高辛本身的浓度无关。我们描述了一种对贝克顿·迪金森固相放射免疫分析法的改进方法,该方法使其更准确、更易于操作,并消除了内源性干扰。地高辛浓度在5.0至至少1000微克/升之间均可测定。

相似文献

1
Pitfalls in, and new methods for, the radioimmunoassay for digoxin in urine.尿地高辛放射免疫测定法中的陷阱及新方法
Clin Chem. 1985 Jan;31(1):122-4.
2
A radioimmunoassay for digoxin in human urine.
J Clin Chem Clin Biochem. 1984 Jun;22(6):403-6. doi: 10.1515/cclm.1984.22.6.403.
3
Factors of importance for valid digitalis assays particularly for the determination of digoxin in plasma and urine.
Acta Pharmacol Toxicol (Copenh). 1986;59 Suppl 4:1-62.
4
A critical appraisal of a further three new commercial digoxin radioimmunoassay kits with reference to cross-reacting substances.对另外三种新的商业化地高辛放射免疫分析试剂盒与交叉反应物质相关的批判性评估。
J Clin Chem Clin Biochem. 1979 Feb;17(2):77-83. doi: 10.1515/cclm.1979.17.2.77.
5
Digoxin-like immunoreactivity in normal human plasma and urine, as detected by a solid-phase radioimmunoassay.
Clin Chem. 1984 Mar;30(3):450-1.
6
[Determination of blood digoxin concentrations by radioimmunoassay--evaluation by SPAC Digoxin Kit].
Kaku Igaku. 1982 Apr;19(3):501-6.
7
Seven different digoxin immunoassay kits compared with respect to interference by a digoxin-like immunoreactive substance in serum from premature and full-term infants.比较七种不同的地高辛免疫分析试剂盒,观察早产儿和足月儿血清中地高辛样免疫反应物质的干扰情况。
Clin Chem. 1983 Nov;29(11):1972-4.
8
[Determination of serum digoxin concentration by digoxin-125I-Imusay diagnostic kit (author's transl)].
Kaku Igaku. 1978 Dec;15(8):1175-82.
9
[Radioimmunoassay of blood digoxin contents--experimental evaluation and clinical application of a method using an Amerlex anti-digoxin serum suspension].
Kaku Igaku. 1982 Aug;19(7):1069-76.
10
[Basic evaluation of a magnetic digoxin radioimmunoassay kit].
Radioisotopes. 1985 May;34(5):274-7. doi: 10.3769/radioisotopes.34.5_274.

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The digoxin-amiodarone interaction.
Cardiovasc Drugs Ther. 1989 Mar;3(1):25-8. doi: 10.1007/BF01881526.