Lindstedt G, Olbe L, Kilander A F, Armbrecht U, Jagenburg R, Runsteen D, Lundberg P A
Clin Chem. 1985 Jan;31(1):76-82.
Prior to a study of the pathophysiological significance of chronic atrophic gastritis and hypergastrinemia, we evaluated a new radioimmunoassay kit for serum total gastrin (Diagnostic Products Corp.). The mean intra-assay CV ranged from 2 to 5% (2386 patients' samples in 47 assay runs done during four months). Total CVs for two controls ranged from 4 to 10%. Within-assay bias was 5%. Oleate decreased the values, indicating that intravenous heparin, which releases endothelial lipase, causing in vitro lipolysis, should be avoided if indwelling catheters are used for sampling, e.g., during provocation tests for gastrin release. Of three other commercial kits examined, two were affected by oleate. Other anions such as heparin and EDTA also affected the assay. Values for gastrin in heparinized plasma from surgical patients representing a variety of disorders agreed well with results obtained by a reference laboratory. We confirm the usefulness of this assay for discriminating clinical situations and conclude that ligand assays, besides those for thyroid assessment, should be assessed for interference from nonesterified fatty acids. Preliminary data also suggest a marked age dependence of serum gastrin concentration.
在研究慢性萎缩性胃炎和高胃泌素血症的病理生理意义之前，我们评估了一种用于血清总胃泌素的新型放射免疫分析试剂盒（诊断产品公司）。批内变异系数均值在2%至5%之间（四个月内进行的47次检测中对2386例患者样本进行检测）。两个对照的总变异系数在4%至10%之间。批内偏差为5%。油酸会使检测值降低，这表明如果使用留置导管采样，例如在胃泌素释放激发试验期间，应避免使用静脉注射肝素，因为肝素会释放内皮脂肪酶，导致体外脂肪分解。在所检测的其他三种商业试剂盒中，有两种受油酸影响。肝素和乙二胺四乙酸等其他阴离子也会影响该检测。代表各种疾病的外科手术患者的肝素化血浆中的胃泌素值与参考实验室获得的结果非常一致。我们证实了该检测在区分临床情况方面的有用性，并得出结论，除甲状腺评估检测外，配体检测也应评估非酯化脂肪酸的干扰情况。初步数据还表明血清胃泌素浓度存在明显的年龄依赖性。
