Jin Zi-Lan, Wang Wan-Ni, Nan Qiong, Liu Jia-Wen, Ju Yan-Lun, Fang Yu-Lin
College of Enology, Northwest A & F University, Yangling, 712100, Shaanxi, China.
College of Enology, Northwest A & F University, Yangling, 712100, Shaanxi, China.
Plant Physiol Biochem. 2025 Feb;219:109379. doi: 10.1016/j.plaphy.2024.109379. Epub 2024 Dec 4.
NAC transcription factors are unique to plants and play a role in stress. In this study, the VvNAC17 gene was isolated from grapes, and tested the functions of VvNAC17 under drought stress. The expression level of VvNAC17 in callus could be highly induced by drought stress. VvNAC17 overexpression in callus conferred drought-resistant phenotypes with lower MDA content, higher antioxidant enzyme activity (CAT, POD, SOD), higher monomer anthocyanin content, and higher expression levels of some drought-related genes (VvDREB1A, VvDREB2A, VvDREB2D, VvRD29A, VvPIN5) and anthocyanin-biosynthesis-related genes (VvUFGT, VvANS, VvANR, VvDFR,VvLAR). Meanwhile, the Y1H and Dual-LUC assays showed that VvNAC17 could activate VvDREB1A and VvUFGT expression by binding to its promoter. Futhermore, RNA-seq showed that VvNAC17 can affect grape growth and development by affecting the photosynthesis and metabolism of some macromolecules. Taken together, the NAC transcription factor VvNAC17 could positively regulates drought-tolerance. VvNAC17 is a promising candidate for improving drought resistance in grape.
NAC转录因子是植物特有的，在胁迫中发挥作用。在本研究中，从葡萄中分离出VvNAC17基因，并测试了VvNAC17在干旱胁迫下的功能。干旱胁迫可高度诱导愈伤组织中VvNAC17的表达水平。愈伤组织中VvNAC17的过表达赋予了抗旱表型，表现为丙二醛含量较低、抗氧化酶活性（CAT、POD、SOD）较高、单体花青素含量较高，以及一些干旱相关基因（VvDREB1A、VvDREB2A、VvDREB2D、VvRD29A、VvPIN5）和花青素生物合成相关基因（VvUFGT、VvANS、VvANR、VvDFR、VvLAR）的表达水平较高。同时，酵母单杂交和双荧光素酶实验表明，VvNAC17可以通过与其启动子结合来激活VvDREB1A和VvUFGT的表达。此外，RNA测序表明，VvNAC17可以通过影响一些大分子的光合作用和代谢来影响葡萄的生长发育。综上所述，NAC转录因子VvNAC17可以正向调节耐旱性。VvNAC成员是提高葡萄抗旱性的一个有潜力的候选基因。
