Gillespie L L, Armstrong J B, Steinberg M S
Dev Biol. 1985 Jan;107(1):220-6. doi: 10.1016/0012-1606(85)90390-2.
Mesoderm of axolotl embryos at various developmental stages was briefly exposed to a calcium-free 0.01% trypsin solution by temporary removal of the epidermis. This treatment was found to disrupt somite segmentation in a localized region and the pronephric duct was unable to migrate through this region. The affected area, consisting of 3.91 +/- 1.04 somites, traveled through the embryo in synchrony with, and 3.55 +/- 0.69-somite widths ahead of segmentation. Trypsinization in the presence of 340 microM calcium resulted in normal duct migration while somite segmentation was still affected. These results demonstrate the existence of a trypsin-sensitive region in the somitic mesoderm and the lateral mesoderm of the duct path that travels in advance of somite segmentation and in synchrony with it. In addition, the trypsin sensitivity of the duct path is calcium dependent whereas that of the somitic mesoderm is not.
通过暂时去除表皮，将不同发育阶段的蝾螈胚胎中胚层短暂暴露于无钙的0.01%胰蛋白酶溶液中。发现这种处理会破坏局部区域的体节分割，并且原肾管无法穿过该区域。受影响区域由3.91±1.04个体节组成，与分割同步并在分割前3.55±0.69个体节宽度处穿过胚胎。在存在340微摩尔钙的情况下进行胰蛋白酶处理会导致导管正常迁移，而体节分割仍受影响。这些结果表明，在体节中胚层和导管路径的外侧中胚层中存在一个对胰蛋白酶敏感的区域，该区域在体节分割之前并与其同步移动。此外，导管路径的胰蛋白酶敏感性是钙依赖性的，而体节中胚层的则不是。
