Sundrani Deepali, Kapare Aishwarya, Yadav Himanshi, Randhir Karuna, Gupte Sanjay, Joshi Sadhana
Mother and Child Health, ICMR - Collaborating Centre of Excellence (CCoE), Interactive Research School for Health Affairs, Bharati Vidyapeeth (Deemed to be University), Pune, Maharashtra, India.
Department of Obstetrics and Gynecology, Gupte Hospital and Research Centre, Pune, Maharashtra, India.
Epigenomics. 2025 Jan;17(1):21-31. doi: 10.1080/17501911.2024.2438593. Epub 2024 Dec 10.
This study aims to examine the gene expression and DNA methylation patterns of angiogenic factors in the placentae of Indian women who underwent assisted reproductive technology (ART) procedures and their association with maternal one-carbon metabolites and birth outcome.
Placental gene expression and DNA methylation of angiogenic factors (, , , ) in Indian women who underwent ART procedures ( = 64) and women who conceived naturally (Non-ART) ( = 93) was investigated using RT-qPCR and Epitect Methyl-II PCR assay kits. Maternal plasma one-carbon metabolites were assessed by CMIA technology.
Gene expression of and was higher ( < 0.05) in the ART placentae. Placental global DNA methylation levels were higher ( < 0.01) and DNA methylation levels of promoter were lower ( < 0.05) in ART compared to non-ART women. Maternal plasma folate and vitamin B levels were higher ( < 0.01) in the ART group. Gene expression of was negatively associated with maternal plasma folate ( < 0.05) whereas KDR was positively associated with maternal plasma homocysteine ( < 0.05). Gene expression of was positively associated with chest circumference of the baby ( < 0.05).
Hypomethylation of and increased expression of and was observed in the placentae of women who underwent ART procedure.
本研究旨在检测接受辅助生殖技术（ART）的印度女性胎盘血管生成因子的基因表达和DNA甲基化模式，及其与母体一碳代谢物和出生结局的关联。
采用RT-qPCR和Epitect Methyl-II PCR检测试剂盒，对接受ART的印度女性（n = 64）和自然受孕女性（非ART，n = 93）胎盘血管生成因子（、、、）的基因表达和DNA甲基化情况进行研究。采用CMIA技术评估母体血浆一碳代谢物。
ART胎盘组织中及的基因表达较高（P < 0.05）。与非ART女性相比，ART女性胎盘整体DNA甲基化水平较高（P < 0.01），而启动子的DNA甲基化水平较低（P < 0.05）。ART组母体血浆叶酸和维生素B水平较高（P < 0.01）。的基因表达与母体血浆叶酸呈负相关（P < 0.05），而KDR与母体血浆同型半胱氨酸呈正相关（P < 0.05）。的基因表达与婴儿胸围呈正相关（P < 0.05）。
接受ART的女性胎盘组织中存在低甲基化，且和的表达增加。
