Placental expression and methylation of angiogenic factors in assisted reproductive technology pregnancies from India.

AIM

This study aims to examine the gene expression and DNA methylation patterns of angiogenic factors in the placentae of Indian women who underwent assisted reproductive technology (ART) procedures and their association with maternal one-carbon metabolites and birth outcome.

METHODS

Placental gene expression and DNA methylation of angiogenic factors (, , , ) in Indian women who underwent ART procedures ( = 64) and women who conceived naturally (Non-ART) ( = 93) was investigated using RT-qPCR and Epitect Methyl-II PCR assay kits. Maternal plasma one-carbon metabolites were assessed by CMIA technology.

RESULT

Gene expression of and was higher ( < 0.05) in the ART placentae. Placental global DNA methylation levels were higher ( < 0.01) and DNA methylation levels of promoter were lower ( < 0.05) in ART compared to non-ART women. Maternal plasma folate and vitamin B levels were higher ( < 0.01) in the ART group. Gene expression of was negatively associated with maternal plasma folate ( < 0.05) whereas KDR was positively associated with maternal plasma homocysteine ( < 0.05). Gene expression of was positively associated with chest circumference of the baby ( < 0.05).

CONCLUSION

Hypomethylation of and increased expression of and was observed in the placentae of women who underwent ART procedure.