Bradsher R W, Ulmer W C, Marmer D J, Townsend J W, Jacobs R F
J Infect Dis. 1985 Jan;151(1):57-64. doi: 10.1093/infdis/151.1.57.
Blastomyces dermatitidis evokes responses of human cellular immunity typical of other intracellular fungal pathogens. Differences in growth rates of intracellular Blastomyces yeast and the differences in amounts of yeast phagocytized by macrophages were determined for macrophages derived from peripheral blood monocytes from 11 persons with treated blastomycosis and 11 normal, healthy persons. Cellular immunity was examined by lymphocyte uptake of [3H]thymidine in response to a specific antigen of Blastomyces yeast. Yeast were more readily phagocytized by macrophages from the previously treated donors when compared with those from the normal donors; the yeast were confirmed to be intracellular by transmission electron microscopy. Likewise, a decrease in growth rates of yeast was demonstrable in cultures of macrophages from previously treated donors as compared with normal donors. This greater efficiency of phagocytosis and growth inhibition of B. dermatitidis reflects another mechanism of human cellular immunity to this fungal infection.
皮炎芽生菌引发的人体细胞免疫反应与其他细胞内真菌病原体典型的反应相同。对11例已治愈芽生菌病患者和11名正常健康人的外周血单核细胞来源的巨噬细胞，测定了细胞内皮炎芽生菌酵母的生长速率差异以及巨噬细胞吞噬酵母数量的差异。通过淋巴细胞对[3H]胸腺嘧啶核苷的摄取来检测针对皮炎芽生菌酵母特异性抗原的细胞免疫。与正常供体来源的巨噬细胞相比，先前接受治疗的供体来源的巨噬细胞更容易吞噬酵母；通过透射电子显微镜证实酵母存在于细胞内。同样，与正常供体相比，先前接受治疗的供体来源的巨噬细胞培养物中酵母的生长速率明显降低。这种对皮炎芽生菌更强的吞噬作用和生长抑制反映了人体对这种真菌感染的细胞免疫的另一种机制。
