Pod5Viewer：用于检查原始纳米孔测序数据的图形用户界面。

Pod5Viewer: a GUI for inspecting raw nanopore sequencing data.

作者信息

Dietrich Vincent, Alagna Nicolò, Helm Mark, Gerber Susanne, Butto Tamer

机构信息

Institute of Human Genetics, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, 55128, Germany.

Institute of Pharmaceutical and Biomedical Sciences, Johannes Gutenberg-University Mainz, Mainz, 55128, Germany.

出版信息

Bioinformatics. 2024 Nov 28;40(12). doi: 10.1093/bioinformatics/btae665.

DOI:10.1093/bioinformatics/btae665

PMID:39657315

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11630908/

Abstract

MOTIVATION

Oxford Nanopore Technologies recently adopted the POD5 file format for storing raw nanopore sequencing data. The information stored in these files provides detailed insights into the sequencing features and enhances the understanding of raw nanopore data. However, the process of visualizing the data can be cumbersome, especially for users without programming skills. To address this issue, we developed the pod5Viewer, a GUI application for inspecting POD5 files.

RESULTS

The pod5Viewer offers straightforward access to raw sequencing data and associated metadata in POD5 files. It includes functionalities for viewing, plotting, and exporting individual reads. Designed with user-friendliness in mind, the pod5Viewer is easy to install and use, making it suitable for users with all technical backgrounds.

AVAILABILITY AND IMPLEMENTATION

The pod5Viewer is available as open source from the pod5Viewer Github repository (https://github.com/dietvin/pod5Viewer).

摘要

动机

牛津纳米孔技术公司最近采用了POD5文件格式来存储原始纳米孔测序数据。这些文件中存储的信息提供了对测序特征的详细洞察，并增强了对原始纳米孔数据的理解。然而，数据可视化过程可能很繁琐，尤其是对于没有编程技能的用户。为了解决这个问题，我们开发了pod5Viewer，这是一个用于检查POD5文件的GUI应用程序。