Fukushima Yutaro, Kagami Asuka, Sonoda Hirotaka, Shimokawa Kotomi, Suico Mary Ann, Kai Hirofumi, Shuto Tsuyoshi
Department of Molecular Medicine, Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-Honmachi, Chuo-ku, Kumamoto, 862-0973, Japan; Health Life Science S-HIGO Professional Fellowship Program, Kumamoto University, 2-39-1 Kurokami, Chuo-ku, Kumamoto, 862-8555, Japan.
Department of Molecular Medicine, Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-Honmachi, Chuo-ku, Kumamoto, 862-0973, Japan; Program for Fostering Innovators to Lead a Better Co-being Society, Kumamoto University, 2-39-1 Kurokami, Chuo-ku, Kumamoto, 862-8555, Japan.
Biochem Biophys Res Commun. 2025 Jan;742:151156. doi: 10.1016/j.bbrc.2024.151156. Epub 2024 Dec 7.
Caenorhabditis elegans (C. elegans) is a robust model organism in cell biology, physiology, pharmacology, and toxicology. It is widely recognized for its short lifespan (about 30 days), rapid life cycle, and genetic similarities to mammals. Known for their utility in lifespan research, compounds identified in C. elegans studies have shown lifespan-extending effects in higher organisms, making them invaluable for aging research. Recent work has highlighted the importance of food source conditions, specifically whether C. elegans is fed live or dead Escherichia coli (E. coli) OP50, and solvents like dimethyl sulfoxide (DMSO) in evaluating compound efficacy and organismal health. In this study, we employed C. elegans health lifespan auto-monitoring system (C-HAS), an automated imaging technology capable of objectively analyzing lifespan and healthspan by tracking movement patterns in real-time. Our results reveal that C. elegans fed dead bacteria, specifically heat-killed (HK) and freeze-dried (Fd) E. coli, display extended lifespan and healthspan compared to those fed live bacteria, reducing the proportion of short-lived, unhealthy nematodes. Moreover, 0.1 % DMSO treatment, a concentration previously reported as not affecting nematode longevity, notably shortens both lifespan and healthspan in C. elegans under dead bacterial conditions, with similar negative effects observed across different dead bacteria types. These findings highlight the importance of considering bacterial food state and DMSO presence when conducting lifespan and healthspan studies in C. elegans. This work provides foundational insights into how specific experimental conditions impact the health quality of C. elegans, advancing our understanding of environmental influences on organismal aging.
秀丽隐杆线虫（C. elegans）是细胞生物学、生理学、药理学和毒理学领域中一种强大的模式生物。它因其寿命短（约30天）、生命周期快以及与哺乳动物的基因相似性而被广泛认可。在寿命研究中具有重要作用，在秀丽隐杆线虫研究中鉴定出的化合物已在高等生物中显示出延长寿命的作用，使其在衰老研究中具有极高价值。最近的研究强调了食物来源条件的重要性，特别是秀丽隐杆线虫是喂食活的还是死的大肠杆菌（E. coli）OP50，以及二甲基亚砜（DMSO）等溶剂在评估化合物功效和生物体健康方面的作用。在本研究中，我们采用了秀丽隐杆线虫健康寿命自动监测系统（C-HAS），这是一种能够通过实时跟踪运动模式客观分析寿命和健康寿命的自动化成像技术。我们的结果表明，与喂食活细菌的线虫相比，喂食死细菌（特别是热杀死的（HK）和冻干的（Fd）大肠杆菌）的秀丽隐杆线虫显示出更长的寿命和健康寿命，减少了短命、不健康线虫的比例。此外，0.1% DMSO处理（先前报道该浓度不影响线虫寿命）在死细菌条件下显著缩短了秀丽隐杆线虫的寿命和健康寿命，在不同类型的死细菌中均观察到类似的负面影响。这些发现突出了在秀丽隐杆线虫中进行寿命和健康寿命研究时考虑细菌食物状态和DMSO存在的重要性。这项工作为特定实验条件如何影响秀丽隐杆线虫的健康质量提供了基础见解，推进了我们对环境对生物体衰老影响的理解。
