Wang Kaiting, Huang Shijie, Li Xiangyu, Ma Jiahao, Li Ying, Kong Simeng, Lin Xinchun
State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou, 310000, China; Bamboo Industry Institute, Zhejiang A&F University, Hangzhou, 310000, China.
State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou, 310000, China; Bamboo Industry Institute, Zhejiang A&F University, Hangzhou, 310000, China.
Plant Physiol Biochem. 2025 Feb;219:109392. doi: 10.1016/j.plaphy.2024.109392. Epub 2024 Dec 6.
Bamboo forests are vulnerable to extreme cold, as well as drought caused by declining rainfall or persistent hot, under global climate change. The C-repeat binding factors/dehydration-responsive element binding protein 1s (CBFs/DREB1s) are vital to acquiring tolerance to deal with the changing climate in plants. Herein, we investigated the evolution of CBFs/DREB1s in four temperate or tropical woody bamboos. In Phyllostachys edulis, Hsuehochloa calcarea, Dendrocalamus latiflorus, and Dendrocalamus brandisii, a total of 16, 12, 24, and 22 putative DREB1s were identified and were categorized into nine subclades, from DREB1A to DREB1I. DREB1s members increased with bamboo polyploidization, coinciding with the presence of at least two collinear DREB1s orthologs in different bamboos. It indicates the importance of polyploidization in driving the expansion of DREB1s. Except for the DREB1F, DREB1s of the other subclades showed direct collinearity with their orthologs in Poaceae. Tandemly linked loci of DREB1A, DREB1H, and DREB1B were of concern due to their conserved and inherited relationship in bamboo, and a recent duplication of DREB1A occurred during bamboo speciation. In P. edulis, PeDREB1A3/PeDREB1H1/PeDREB1B3 locus showed sensitivity to cold stimulation, especially for PeDREB1A3 rapidly induced after 0.5-h cold stimulation. PeDREB1A3 was proved as a nuclear-located transcription activator recognizing DRE cis-element. Moreover, overexpression of PeDREB1A3 improved both cold and drought tolerance of Arabidopsis thaliana. It suggested that the neoteric duplication of DREB1As might contribute to bamboo adaptability. DREB1A represents the potential locus for improving agronomic traits in the future. This research provides valuable information for excavating potential genes for bamboo adaptation and will facilitate the research on bamboo breeding for stress tolerance.
在全球气候变化的影响下，竹林极易受到极端寒冷以及降雨减少或持续高温导致的干旱的影响。C-重复结合因子/脱水响应元件结合蛋白1（CBFs/DREB1s）对于植物获得应对气候变化的耐受性至关重要。在此，我们研究了四种温带或热带木本竹子中CBFs/DREB1s的进化情况。在毛竹、寒竹、麻竹和勃氏甜龙竹中，分别鉴定出16个、12个、24个和22个假定的DREB1s，并将它们分为从DREB1A到DREB1I的九个亚分支。随着竹子多倍体化，DREB1s成员数量增加，这与不同竹子中至少存在两个共线的DREB1s直系同源物相一致。这表明多倍体化在推动DREB1s扩展中的重要性。除了DREB1F外，其他亚分支的DREB1s与其禾本科直系同源物呈现直接共线性。由于DREB1A、DREB1H和DREB1B在竹子中具有保守和遗传关系，其串联连锁位点值得关注，并且在竹子物种形成过程中最近发生了DREB1A的复制。在毛竹中，PeDREB1A3/PeDREB1H1/PeDREB1B3位点对冷刺激敏感，尤其是PeDREB1A3在0.5小时冷刺激后迅速被诱导。PeDREB1A3被证明是一种识别DRE顺式元件的核定位转录激活因子。此外，PeDREB1A3的过表达提高了拟南芥的耐寒性和耐旱性。这表明DREB1As的近期复制可能有助于竹子的适应性。DREB1A代表了未来改善农艺性状的潜在位点。本研究为挖掘竹子适应的潜在基因提供了有价值的信息，并将促进竹子抗逆育种的研究。
