Liu Qian, Shen Hong, Wei Ming, Chen Xi, Gu Li, Zhu Wentao
Department of Clinical Laboratory, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, PR China.
Department of Infectious Diseases and Clinical Microbiology, Beijing Institute of Respiratory Medicine and Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, PR China.
Drug Resist Updat. 2025 Jan;78:101180. doi: 10.1016/j.drup.2024.101180. Epub 2024 Dec 5.
Morganella morganii has been recognized as an important opportunistic pathogen that is becoming increasingly prevalent worldwide. However, the current global evolutionary dynamics and emergence of ARGs remain obscure. The present study determined the global distribution, genomic classification, phylogeny, and monitor longitudinal resistome changes. During 1900-2024, a total of 1027 non-duplicate Morganella genomes have been reported from 49 countries. The countries with the highest number were China (433), the USA (143), and France (74). Through ANI distance analysis and core genome phylogeny, Morganella was reclassified into six species: M. morganii, M. sibonii, M. chanii, M. laugraudii, M. kristinii, M. psychrotolerans. Further analysis using cgMLST identified 87 distinct genetic clusters and 737 singleton strains, indicating a high level of multi-locus sequence type diversity and local clonal outbreaks. Bayesian evolutionary analysis revealed the most recent common ancestor year and potential global transmission routes. A total of 195 ARGs were carried by Morganella isolates, with each genome containing between 2 and 544 ARGs. The most common ARGs were associated with resistance to the following drug-classes: aminoglycosides, beta-lactam, chloramphenicol, sulfamides, and tetracycline. Twenty-one carbapenemase-encoding genes were identified in 22 countries, with bla, bla, bla, bla, bla, bla, and bla being the most prevalent. Positive correlations were observed between ARGs and mobile genetic elements, like plasmids, ISs, and Tns, indicating frequent mobilization of certain ARGs by different mobile genetic elements (p < 0.05). In conclusion, Morganella isolates that are showing an upward trend in resistance and infection rates warrant a reclassification of their taxonomy and continuous monitoring for resistance.
摩根氏摩根菌已被公认为一种重要的机会致病菌，在全球范围内日益普遍。然而，目前全球范围内该菌的进化动态以及抗菌耐药基因（ARGs）的出现情况仍不清楚。本研究确定了其全球分布、基因组分类、系统发育，并监测了纵向耐药组变化。在1900年至2024年期间，共报告了来自49个国家的1027个非重复摩根氏菌基因组。数量最多的国家是中国（433个）、美国（143个）和法国（74个）。通过平均核苷酸一致性（ANI）距离分析和核心基因组系统发育分析，摩根氏菌被重新分类为六个物种：摩根氏摩根菌、西博尼摩根菌、查尼摩根菌、劳格劳迪摩根菌、克里斯蒂尼摩根菌、耐冷摩根菌。使用核心基因组多位点序列分型（cgMLST）的进一步分析确定了87个不同的基因簇和737个单株菌株，表明多位点序列类型具有高度多样性以及存在局部克隆爆发。贝叶斯进化分析揭示了最近共同祖先的年份以及潜在的全球传播途径。摩根氏菌分离株共携带195种ARGs，每个基因组包含2至544种ARGs。最常见的ARGs与对以下药物类别的耐药性相关：氨基糖苷类、β-内酰胺类、氯霉素、磺胺类和四环素类。在22个国家鉴定出21种碳青霉烯酶编码基因，其中bla、bla、bla、bla、bla、bla和bla最为普遍。观察到ARGs与移动遗传元件（如质粒、插入序列和转座子）之间存在正相关，表明某些ARGs通过不同的移动遗传元件频繁移动（p < 0.05）。总之，耐药率和感染率呈上升趋势的摩根氏菌分离株需要对其分类进行重新评估，并持续监测耐药情况。
