Elguindi A S, Smith J K, Speir W A
Life Sci. 1985 Jan 7;36(1):75-81. doi: 10.1016/0024-3205(85)90288-7.
The facilitating effect of nifedipine on isoproterenol induced airway smooth muscle relaxation was studied in guinea pig tracheas. For isometric force measurement, 4 mm tracheal cylinders were suspended in incubation chambers in oxygenated physiologic medium. After 90 minutes of equilibration under 2 grams resting tension, at a temperature of 37 degrees C and pH of 7.4, concentration response curves for isoproterenol were performed with and without the addition of a 1 X 10(-5)M nifedipine dose. The experiments were then repeated using tissues precontracted with histamine. Our data show that in the nifedipine pretreated tissues, the EC50 of isoproterenol is shifted to the left (p less than 0.05) probably due to further reduction in cytosolic calcium by nifedipine. Our findings suggest that nifedipine might have a role in the treatment of asthma and obstructive airway disease.
在豚鼠气管中研究了硝苯地平对异丙肾上腺素诱导的气道平滑肌舒张的促进作用。对于等长力测量，将4毫米气管环悬挂在充满氧气的生理介质的孵育室中。在2克静息张力下平衡90分钟后，在37℃温度和pH 7.4条件下，分别在添加和不添加1×10(-5)M硝苯地平剂量的情况下进行异丙肾上腺素的浓度反应曲线实验。然后使用组胺预收缩的组织重复实验。我们的数据表明，在硝苯地平预处理的组织中，异丙肾上腺素的EC50向左移动（p<0.05），这可能是由于硝苯地平进一步降低了细胞溶质钙。我们的研究结果表明，硝苯地平可能在哮喘和阻塞性气道疾病的治疗中发挥作用。
