Han Changhee, Kim Juil
Interdisciplinary Graduate Program in Smart Agriculture, Kangwon National University, Chuncheon, Republic of Korea.
Agriculture and Life Sciences Research Institute, Kangwon National University, Chuncheon, Republic of Korea.
Heliyon. 2024 Nov 22;10(23):e40556. doi: 10.1016/j.heliyon.2024.e40556. eCollection 2024 Dec 15.
Since the commercialization of diamide insecticides, including chlorantraniliprole, in 2007, the overuse of diamide insecticides for over a decade has resulted in excessive chlorantraniliprole resistance in , causing continuous economic losses. While RyR target-site mutations and detoxification enzymes such as cytochrome P450 have been studied as the leading causes of resistance, previous studies, including functional research and synergistic tests, have not confirmed a clear correlation between these factors and the development of resistance. Thus, transcriptome analysis was employed to investigate alternative strategies beyond mutation(s) in RyR or metabolic factors involving detoxification pathways that allow diamide-resistance to counteract the calcium ion imbalances induced by chlorantraniliprole effectively. Diamide-resistant, susceptible strains and its F1-hybrid of were used for the RNAseq-based differentially expressed gene (DEG) analysis. In total 4669 genes were differentially expressed, with 2809 upregulated and 1860 downregulated in the resistant strain compared to the susceptible strain. GO, KEGG enrichment and orthologous analyses demonstrated that genes involved in metabolic factors were overrepresented in the resistant strain. In particular, overexpressed endoplasmic reticulum (ER)-related calcium ion homeostasis and cell stability-associated genes were newly identified in resistant strain. The selected differentially expressed genes were validated then with qPCR. These genes were inferred to induce cell stability to overcome ER stress derived from calcium ion imbalance caused by chlorantraniliprole. These results provide advanced insights into the critical roles of calcium ion homeostasis- and cell stability-related genes in conferring diamide insecticide resistance.
自2007年双酰胺类杀虫剂（包括氯虫苯甲酰胺）商业化以来，十多年来双酰胺类杀虫剂的过度使用已导致 对氯虫苯甲酰胺产生过度抗性，造成持续的经济损失。虽然已研究了RyR靶位点突变和细胞色素P450等解毒酶作为抗性的主要原因，但包括功能研究和增效试验在内的先前研究尚未证实这些因素与抗性发展之间存在明确的相关性。因此，采用转录组分析来研究除RyR突变或涉及解毒途径的代谢因素之外的其他策略，这些策略可使双酰胺抗性有效抵消氯虫苯甲酰胺诱导的钙离子失衡。使用双酰胺抗性品系、敏感品系及其F1杂交种进行基于RNAseq的差异表达基因（DEG）分析。与敏感品系相比，抗性品系中共有4669个基因差异表达，其中2809个上调，1860个下调。GO、KEGG富集和直系同源分析表明，参与代谢因素的基因在抗性品系中过度表达。特别是，在抗性品系中新发现了内质网（ER）相关钙离子稳态和细胞稳定性相关基因的过表达。然后用qPCR验证所选的差异表达基因。推断这些基因可诱导细胞稳定性以克服由氯虫苯甲酰胺引起的钙离子失衡所产生的内质网应激。这些结果为钙离子稳态和细胞稳定性相关基因在赋予双酰胺类杀虫剂抗性中的关键作用提供了深入见解。
