He Jinjun, Tian Weiyi, Meng Yonghui, Yan An, Lai Xin, Wang Fei, Che Bangwei
Department of Urology & Andrology, The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang, 550001, China.
The First Clinical College, Guizhou University of Traditional Chinese Medicine, Guiyang, 550001, China.
Heliyon. 2024 Nov 23;10(23):e40674. doi: 10.1016/j.heliyon.2024.e40674. eCollection 2024 Dec 15.
The purpose of this study was to investigate the protective effect of xylosma congesta extract on kidney injury in hyperuricemic rats.
The rats were fed yeast extract and intraperitoneal injections of potassium oxonate for 3 weeks to establish the hyperuricemia model. And then the rats were treated with allopurinol and different doses of oak extract. The contents of uric acid in urine and serum, creatinine, and urea nitrogen in serum were detected by biochemical methods. TUNEL was used to detect cell apoptosis in renal tissue. The protein expression of TLR4 and NF- kappa B (NF-κB) p65 and the proportion of CD68 and CD206 positive cells in renal tissue were detected by pathological method.
The xylosma congesta group showed decreased renal tubular dilatation, decreased renal interstitial inflammatory cell infiltration, decreased serum creatinine content, and decreased apoptotic cell count as compared to the model group. And positive expression of TLR4 and NF-κB decreased with each dose. Additionally, the xylosma congesta groups showed a significant rise in CD206 and a considerable decrease in CD68.
The extract from xylosma congesta has the ability to lower serum uric acid and creatinine levels while also providing protection against kidney damage caused by hyperuricemia.
本研究旨在探讨柞木提取物对高尿酸血症大鼠肾损伤的保护作用。
用酵母提取物喂养大鼠，并腹腔注射氧嗪酸钾3周以建立高尿酸血症模型。然后用别嘌醇和不同剂量的柞木提取物对大鼠进行治疗。采用生化方法检测尿液和血清中的尿酸含量、血清肌酐和尿素氮。用TUNEL法检测肾组织中的细胞凋亡。采用病理方法检测肾组织中TLR4和NF-κB p65的蛋白表达以及CD68和CD206阳性细胞的比例。
与模型组相比，柞木组肾小管扩张减轻，肾间质炎性细胞浸润减少，血清肌酐含量降低，凋亡细胞计数减少。TLR4和NF-κB的阳性表达随剂量增加而降低。此外，柞木组CD206显著升高，CD68显著降低。
柞木提取物具有降低血清尿酸和肌酐水平的能力，同时还能保护肾脏免受高尿酸血症引起的损伤。
