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布拉氏酵母菌CNCM I-745对肠道的松弛特性中的5-羟色胺能作用机制

Serotoninergic Mechanisms of Action in the Relaxant Properties of Saccharomyces boulardii CNCM I-745 on the Intestine.

作者信息

Girard P, Verleye M, Castagné V

机构信息

Biocodex - Research and Development Center, Compiègne, France.

出版信息

Dig Dis Sci. 2025 Jan;70(1):191-202. doi: 10.1007/s10620-024-08786-y. Epub 2024 Dec 11.

DOI:10.1007/s10620-024-08786-y
PMID:39661276
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11761163/
Abstract

BACKGROUND

Perturbations of intestinal serotonergic neurotransmission seem to be involved in bowel dysmotility associated with irritable bowel syndrome (IBS) with diarrhea. Oral administration of probiotics is an emerging strategy to improve IBS symptoms, possibly via influencing local serotonin metabolism and neurotransmission. In the present study, we evaluated the effects of the yeast Saccharomyces boulardii CNCM I-745 (S. boulardii) on intestinal motility and serotonergic receptors.

METHODS

Isolated rat ileum was contracted in a cumulative concentration way by serotonin (5-HT), various 5-HT agonists or by acetylcholine to determine their effective concentration 50% (EC). Single concentrations of S. boulardii or 5-HT antagonists were added before agonists to identify the receptors targeted by S. boulardii.

RESULTS

The serotonin antagonists 5-HT WAY100635, 5-HT ketanserin and 5-HT GR113808 inhibited 5-HT-induced contractions in a concentration-dependent manner. S. boulardii between 0.05 and 1.5 mg/mL increased the EC value of 5-HT suggesting an inhibitory effect against serotonin-induced contraction. Ileum contractions induced by the serotonin agonist 5-HT carboxamidotryptamine or by the serotonin agonist 5-HT alpha-methyl-5-HT were significantly reduced by S. boulardii at 1.5 mg/mL. The yeast did not affect acetylcholine-induced ileum contraction.

CONCLUSION

S. boulardii CNCM I-745 possesses relaxant properties on the rat ileum involving the inhibition of 5-HT and more specifically 5-HT and 5-HT receptor-induced contractions. These data suggest that the attenuation of 5-HT-induced ileal contractions by S. boulardii represents a probable mechanism of action sustaining its efficacy in patients affected by IBS with diarrhea.

摘要

背景

肠道5-羟色胺能神经传递的紊乱似乎与腹泻型肠易激综合征（IBS）相关的肠道运动障碍有关。口服益生菌是一种新兴的改善IBS症状的策略，可能是通过影响局部5-羟色胺代谢和神经传递来实现。在本研究中，我们评估了布拉酵母菌CNCM I-745（S. boulardii）对肠道运动和5-羟色胺能受体的影响。

方法

用5-羟色胺（5-HT）、各种5-HT激动剂或乙酰胆碱以累积浓度的方式使离体大鼠回肠收缩，以确定它们的半数有效浓度（EC）。在激动剂之前加入单一浓度的S. boulardii或5-HT拮抗剂，以确定S. boulardii作用的受体。

结果

5-羟色胺拮抗剂5-HT WAY100635、5-HT酮色林和5-HT GR113808以浓度依赖性方式抑制5-HT诱导的收缩。0.05至1.5mg/mL的S. boulardii增加了5-HT的EC值，表明对5-羟色胺诱导的收缩有抑制作用。1.5mg/mL的S. boulardii显著降低了5-羟色胺激动剂5-羧酰胺色胺或5-羟色胺激动剂5-甲基-5-羟色胺诱导的回肠收缩。该酵母不影响乙酰胆碱诱导的回肠收缩。

结论

S. boulardii CNCM I-745对大鼠回肠具有舒张特性，涉及对5-HT，更具体地说是对5-HT和5-HT受体诱导的收缩的抑制。这些数据表明，S. boulardii对5-羟色胺诱导的回肠收缩的减弱是维持其对腹泻型IBS患者疗效的一种可能作用机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9300/11761163/fab35ca6fbaa/10620_2024_8786_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9300/11761163/1a3a5989095b/10620_2024_8786_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9300/11761163/fab35ca6fbaa/10620_2024_8786_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9300/11761163/1a3a5989095b/10620_2024_8786_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9300/11761163/fab35ca6fbaa/10620_2024_8786_Fig2_HTML.jpg

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本文引用的文献

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Curr Ther Res Clin Exp. 2023 Oct 18;99:100721. doi: 10.1016/j.curtheres.2023.100721. eCollection 2023.
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The Effects of Serotonin Receptor Type 7 Modulation on Bowel Sensitivity and Smooth Muscle Tone in Patients With Irritable Bowel Syndrome.5-羟色胺7型受体调节对肠易激综合征患者肠道敏感性和平滑肌张力的影响
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Saccharomyces boulardii, a yeast probiotic, inhibits gut motility through upregulating intestinal serotonin transporter and modulating gut microbiota.布拉氏酵母菌，一种酵母益生菌，通过上调肠道 5-羟色胺转运体和调节肠道微生物群来抑制肠道蠕动。
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