Fu Pengcheng, Zhang Yongqing, Wang Siming, Ye Xin, Wu Yunhong, Yu Mengfei, Zhu Shiyao, Lee Hyeon Jeong, Zhang Delong
Zhejiang Key Laboratory of Micro-nano Quantum Chips and Quantum Control, School of Physics, Zhejiang University, Hangzhou 310027, China.
Key Laboratory of Oral Biomedical Research of Zhejiang Province, Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Cancer Center of Zhejiang University, Hangzhou 310006, China.
Sci Adv. 2024 Dec 13;10(50):eadm7687. doi: 10.1126/sciadv.adm7687. Epub 2024 Dec 11.
Molecular spectroscopy provides intrinsic contrast for in situ chemical imaging, linking the physiochemical properties of biomolecules to the functions of living systems. While stimulated Raman imaging has found successes in deciphering biological machinery, many vibrational modes are Raman inactive or weak, limiting the broader impact of the technique. It can potentially be mitigated by the spectral complementarity from infrared (IR) spectroscopy. However, the vastly different optical windows make it challenging to develop such a platform. Here, we introduce in situ pump-probe IR and Raman excitation (INSPIRE) microscopy, a nascent cross-modality spectroscopic imaging approach by encoding the ultrafast Raman and the IR photothermal relaxation into a single probe beam for simultaneous detection. INSPIRE inherits the merits of complementary modalities and demonstrates high-content molecular imaging of chemicals, cells, tissues, and organisms. Furthermore, INSPIRE applies to label-free and molecular tag imaging, offering possibilities for optical sensing and imaging in biomedicine and materials science.
分子光谱为原位化学成像提供了内在对比度，将生物分子的物理化学性质与生命系统的功能联系起来。虽然受激拉曼成像在解析生物机制方面取得了成功，但许多振动模式是拉曼非活性或微弱的，限制了该技术的更广泛影响。红外（IR）光谱的光谱互补性可能会缓解这一问题。然而，巨大不同的光学窗口使得开发这样一个平台具有挑战性。在此，我们引入原位泵浦-探测红外和拉曼激发（INSPIRE）显微镜，这是一种新兴的交叉模态光谱成像方法，通过将超快拉曼和红外光热弛豫编码到单个探测光束中进行同时检测。INSPIRE继承了互补模态的优点，并展示了对化学物质、细胞、组织和生物体的高内涵分子成像。此外，INSPIRE适用于无标记和分子标签成像，为生物医学和材料科学中的光学传感和成像提供了可能性。
