Fayer Leonara, Vasconcellos Rebecca, de Oliveira Eduarda Rocha, da Silva Almeida Ferreira Caroline, de Souza Nelson Luis Gonçalves Dias, Manhabosco Taíse Matte, de Oliveira Luiz Fernando Cappa, Martins Maria Alice, Brandão Humberto de Mello, Munk Michele
Laboratory of Nanobiotechnology and Nanotoxicology, Department of Biology, Federal University of Juiz de Fora, 36036-900 Juiz de Fora, Brazil.
Collegiate of Exact and Biotechnology Sciences, Federal University of Tocantis, Gurupi 77402-970, Brazil.
Biomed Mater. 2024 Dec 19;20(1). doi: 10.1088/1748-605X/ad9da4.
Chitosan (CS) is a promising polymeric biomaterial for use in scaffolds forskin models and wound dressings, owing to its non-antigenic and antimicrobial properties. However, CS often exhibits insufficient physicochemical properties, mechanical strength, and bioactivity, limiting its efficacy in demanding applications. To address these challenges, cotton cellulose nanofibers (CNFs) represent a promising nanomaterial for enhancing CS-based scaffolds in tissue engineering. CNF offers superior stiffness, and mechanical properties that enhance cellular adhesion and proliferation, both crucial for effective tissue regeneration and healing. This study aimed to develop and characterize a scaffold combining cotton CNF and CS, focusing on its cytocompatibility with human fibroblasts and keratinocytes. The cotton CNF/CS scaffold was fabricated using the casting technique, and its physicochemical properties and cellular compatibility were assessed. The results demonstrated that incorporating cotton CNF significantly enhanced the stability of the CS matrix. The CS scaffold with 1000 μg mlof cotton CNF exhibited increased roughness and reduced rupture strain compared to the pure CS scaffold. The cotton CNF/CS scaffold effectively promoted the adhesion, viability, proliferation, migration, and collagen synthesis of skin cells. Notably, increased cell viability was observed in human fibroblasts cultured on scaffolds with higher concentrations of cotton CNF (100 and 1000 μg ml). Based on the findings, the cotton CNF/CS scaffold demonstrates enhanced physicochemical properties and bioactivity, making it a promising candidate for the development ofhuman skin models and wound healing dressings.
壳聚糖（CS）是一种很有前景的聚合物生物材料，因其具有非抗原性和抗菌特性，可用于皮肤模型支架和伤口敷料。然而，CS的物理化学性质、机械强度和生物活性往往不足，限制了其在苛刻应用中的效果。为应对这些挑战，棉纤维素纳米纤维（CNF）是一种很有前景的纳米材料，可用于增强组织工程中基于CS的支架。CNF具有卓越的刚度和机械性能，可增强细胞黏附和增殖，这对有效的组织再生和愈合都至关重要。本研究旨在开发并表征一种结合棉CNF和CS的支架，重点关注其与人成纤维细胞和角质形成细胞的细胞相容性。采用浇铸技术制备了棉CNF/CS支架，并评估了其物理化学性质和细胞相容性。结果表明，加入棉CNF可显著提高CS基质的稳定性。与纯CS支架相比，含有1000μg/ml棉CNF的CS支架粗糙度增加，断裂应变降低。棉CNF/CS支架有效地促进了皮肤细胞的黏附、活力、增殖、迁移和胶原蛋白合成。值得注意的是，在含有较高浓度棉CNF（100和1000μg/ml）的支架上培养的人成纤维细胞中观察到细胞活力增加。基于这些发现，棉CNF/CS支架显示出增强的物理化学性质和生物活性，使其成为开发人类皮肤模型和伤口愈合敷料的有前景的候选材料。
