褪黑素处理通过调节哈密瓜采后碳水化合物代谢来延缓果实软化。

Melatonin treatment delayed fruit softening by regulating postharvest carbohydrate metabolism of hami melon.

作者信息

Wang Yue, Liu Guang, Zhang Weida, Li Lingling, Yang Wanting, Liu Yuxing, Jiang Li, Guo Minrui, Chen Guogang

机构信息

College of Food Science and Technology, Shihezi University, Shihezi, Xinjiang, 832000, China; Research Center of Xinjiang Characteristic Fruit and Vegetable Storage and Processing Engineering, Ministry of Education, Shihezi, Xinjiang, 832000, China.

Institute of Vegetable Crop, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.

出版信息

Plant Physiol Biochem. 2025 Feb;219:109328. doi: 10.1016/j.plaphy.2024.109328. Epub 2024 Dec 3.

DOI:10.1016/j.plaphy.2024.109328

PMID:39662392

Abstract

Melatonin (MT) treatment has been proven to improve fruit quality. Herein, the efficacy of 0.5 mmol L MT treatment on carbohydrate metabolism, fruit softening, and their relationship during storage of harvested Hami melons was investigated. The results indicated that compared with those in control group, MT treatment markedly delayed fruit softening and the total soluble solids/titratable acid ratio, inhibited cell wall polysaccharide degradation, and maintained the cell wall integrity in Hami melons. Besides, MT treated also increased the starch, sucrose, and sorbitol contents, enhanced sucrose phosphate synthase and sucrose synthase synthesis activities, whereas reduced pectin methyl esterase, polygalacturonase, cellulase, β-galactosidase, β-glucosidase, xyloglucan endotransglycosylase, amylase, sucrose synthase-cleavage, acid invertase, neutral invertase, sorbitol oxidase, NAD-sorbitol dehydrogenase, NADP-sorbitol dehydrogenase activities in Hami melons. Additionally, MT treatment downregulated the expression levels of CmPG, Cmβ-gal, CmAmy, CmAI, CmNI and CmSOX, and upregulated the expression of CmSPS. Collectively, MT could slow fruit softening and prolong its shelf life by regulating carbohydrate metabolism.

摘要

褪黑素（MT）处理已被证明可改善果实品质。在此，研究了0.5 mmol/L MT处理对采收后哈密瓜贮藏期间碳水化合物代谢、果实软化及其关系的影响。结果表明，与对照组相比，MT处理显著延缓了果实软化和总可溶性固形物/可滴定酸比值，抑制了细胞壁多糖降解，并维持了哈密瓜细胞壁的完整性。此外，MT处理还增加了淀粉、蔗糖和山梨醇含量，增强了蔗糖磷酸合酶和蔗糖合酶的合成活性，而降低了哈密瓜中果胶甲酯酶、多聚半乳糖醛酸酶、纤维素酶、β-半乳糖苷酶、β-葡萄糖苷酶、木葡聚糖内转糖基酶、淀粉酶、蔗糖合酶裂解酶、酸性转化酶、中性转化酶、山梨醇氧化酶、NAD-山梨醇脱氢酶、NADP-山梨醇脱氢酶的活性。此外，MT处理下调了CmPG、Cmβ-gal、CmAmy、CmAI、CmNI和CmSOX的表达水平，并上调了CmSPS的表达。总体而言，MT可通过调节碳水化合物代谢来减缓果实软化并延长其货架期。