Liao Fengping, Xu Xin, Wang Hanxu, Huang Linru, Li Kuntai
College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Province Engineering Laboratory for Marine Biological Products, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, Zhanjiang 524088, China.
College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Province Engineering Laboratory for Marine Biological Products, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, Zhanjiang 524088, China.
Int J Biol Macromol. 2025 Jan;287:138583. doi: 10.1016/j.ijbiomac.2024.138583. Epub 2024 Dec 9.
This study aimed to clarify the antifungal activity and action mechanism of the lipopeptides from a marine Bacillus amyloliquefaciens HY2-1 against Penicillium digitatum both in vitro and in vivo. Results showed that HY2-1 lipopeptides exerted obvious inhibitions on spore germination and mycelium growth of P. digitatum. Furthermore, HY2-1 lipopeptides caused the aggravated lipid peroxidation, the decreased ergosterol biosynthesis, and the increased nucleic acid and protein leakage in P. digitatum, suggesting the damaged membrane integrity and permeability of P. digitatum. In addition, HY2-1 lipopeptides induced ROS burst and reduced the activities of antioxidant superoxide dismutase and peroxidase in P. digitatum, indicating that the intracellular redox homeostasis of P. digitatum was disturbed. In vivo biocontrol experiments showed that HY2-1 lipopeptides could effectively reduce the occurrence of green mold disease in citrus fruits artificially inoculated with P. digitatum spores. Most significantly, 4.8 mg/mL of HY2-1 lipopeptides achieved 100 % of biocontrol efficacy after 30 days of storage and increased significantly contents of total phenols and flavonoids in citrus peels, demonstrating that HY2-1 lipopeptides inhibited green mold disease by exerting their antifungal activities and triggering fruit defense responses. This study deepens the understanding of marine Bacillus lipopeptides in the biological control of postharvest diseases.
本研究旨在阐明海洋解淀粉芽孢杆菌HY2-1产生的脂肽在体外和体内对指状青霉的抗真菌活性及作用机制。结果表明，HY2-1脂肽对指状青霉的孢子萌发和菌丝生长有明显抑制作用。此外，HY2-1脂肽导致指状青霉脂质过氧化加剧、麦角甾醇生物合成减少以及核酸和蛋白质泄漏增加，表明指状青霉的细胞膜完整性和通透性受损。此外，HY2-1脂肽诱导指状青霉产生ROS爆发，并降低其抗氧化超氧化物歧化酶和过氧化物酶的活性，表明指状青霉的细胞内氧化还原稳态受到干扰。体内生物防治实验表明，HY2-1脂肽能有效减少人工接种指状青霉孢子的柑橘果实上绿霉病的发生。最显著的是，4.8 mg/mL的HY2-1脂肽在储存30天后实现了100%的生物防治效果，并显著提高了柑橘果皮中总酚和类黄酮的含量，表明HY2-1脂肽通过发挥其抗真菌活性和触发果实防御反应来抑制绿霉病。本研究加深了对海洋芽孢杆菌脂肽在采后病害生物防治中的理解。
