Rumanova Valentina Sophia, Foppen Ewout, Okuliarova Monika, Zeman Michal, Kalsbeek Andries
Hypothalamic Integration Mechanisms, Netherlands Institute for Neuroscience (NIN), An Institute of the Royal Netherlands Academy of Arts and Sciences (KNAW), Amsterdam, the Netherlands; Department of Endocrinology and Metabolism, Amsterdam UMC, University of Amsterdam, Amsterdam, the Netherlands; Department of Animal Physiology and Ethology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia; Laboratory of Endocrinology, Amsterdam University Medical Centers, Amsterdam Gastroenterology Endocrinology Metabolism (AGEM), Amsterdam, the Netherlands.
Hypothalamic Integration Mechanisms, Netherlands Institute for Neuroscience (NIN), An Institute of the Royal Netherlands Academy of Arts and Sciences (KNAW), Amsterdam, the Netherlands; Laboratory of Endocrinology, Amsterdam University Medical Centers, Amsterdam Gastroenterology Endocrinology Metabolism (AGEM), Amsterdam, the Netherlands.
Physiol Behav. 2025 Mar 1;290:114780. doi: 10.1016/j.physbeh.2024.114780. Epub 2024 Dec 9.
Exposure to artificial light at night (ALAN) disrupts natural darkness and desynchronizes daily rhythms in physiological processes and behavior. Previously, in rats, we have shown that dim ALAN disturbed the central circadian control and the temporal organization of behavior, and hormonal and metabolic pathways. The measurements of undisturbed daily behavioral (locomotor activity, feeding and drinking) patterns revealed reduced amplitudes and a transitory activity peak in the middle of the light (i.e. resting) period. Recent studies indicated that time-restricted feeding during the active period (TRFd) can strengthen daily rhythms and improve metabolic health. Therefore, the aim of our study was to prevent the dim ALAN-induced attenuation of daily behavioral rhythms by applying TRFd. Male Wistar rats were kept in a 12/12 light/dark cycle in metabolic cages for one week with free access to food and water. After acclimation, rats were divided into two groups: 1) ad libitum food or 2) time-restricted food during the dark period. After one week, both groups were exposed to dim ALAN for two weeks. Despite the enhanced amplitude of the daily feeding rhythm in TRFd animals, ALAN still suppressed the rhythm of locomotor activity, induced the extra peak during the resting period and reduced the bimodal pattern during the night. Furthermore, TRFd did not prevent the drop in anticipatory thirst caused by ALAN at the end of the active period. In conclusion, TRFd was not able to fully prevent the weakning of daily behavioral rhythms by dim ALAN.
夜间暴露于人造光（ALAN）会破坏自然黑暗，并使生理过程和行为中的日常节律失调。此前，我们在大鼠身上已表明，昏暗的ALAN会扰乱中枢昼夜节律控制以及行为、激素和代谢途径的时间组织。对未受干扰的日常行为（运动活动、进食和饮水）模式的测量显示，在光照（即休息）期中间，振幅减小且出现一个短暂的活动峰值。最近的研究表明，在活跃期进行限时进食（TRFd）可以加强日常节律并改善代谢健康。因此，我们研究的目的是通过应用TRFd来防止昏暗的ALAN引起的日常行为节律减弱。雄性Wistar大鼠在代谢笼中饲养于12/12小时光照/黑暗周期中一周，可自由获取食物和水。适应环境后，大鼠被分为两组：1）随意进食组或2）在黑暗期进行限时进食组。一周后，两组均暴露于昏暗的ALAN环境中两周。尽管TRFd组动物的每日进食节律振幅有所增加，但ALAN仍抑制了运动活动节律，在休息期诱导出现额外的峰值，并减少了夜间的双峰模式。此外，TRFd未能防止ALAN在活跃期末尾引起的预期口渴下降。总之，TRFd无法完全防止昏暗的ALAN导致的日常行为节律减弱。
