Zhang Qian, Ke Jin, Cui Guangfu, Qian Shen, Qian Weixin, Moon Sun-Wook, Sun Yanyan, Huang Tianwen, Qin Zaisheng
Department of Anesthesiology, Nanfang Hospital, Southern Medical University, Guangdong Provincial Key Laboratory of Precision Anesthesia and Perioperative Organ Protection, Guangzhou, Guangdong 510515, China; Department of Anesthesiology, Shenzhen University General Hospital and Shenzhen University Academy of Clinical Medical Sciences, Shenzhen University, Shenzhen 518055, China.
CAS Key Laboratory of Brain Connectome and Manipulation, Institute of Brain Cognition and Brain Disease, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong 518055, China; Shenzhen Key Laboratory of Drug Addiction, Shenzhen-Hong Kong Institute of Brain Science, Brain Cognition and Brain Disease Institute, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong 518055, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Neurosci Lett. 2025 Jan 18;846:138080. doi: 10.1016/j.neulet.2024.138080. Epub 2024 Dec 9.
General anesthesia has been widely used in surgical procedures. Propofol and isoflurane are the most commonly used injectable and inhaled anesthetics, respectively. The various adverse effects induced by propofol and isoflurane are highly associated with the anesthetic-dependent change of brain activities. In this work, we aim to delineate a brain-wide neuronal activity landscape of injectable versus inhaled anesthetics to understand the neural basis underlying the different physiological effects induced by these two major types of anesthetics. Through detailed scanning of the whole mouse brain subjected to propofol or isoflurane anesthesia, in total, we identified 17 subcortical regions, 3 of which (anterodorsal preoptic nucleus, ADP; lateral habenular, LHb; inferior olivary nucleus, ION) were specifically activated by propofol, and 3 (ventral part of the lateral septum, LSV; the intermediate part of the lateral septum, LSI; the solitary tract nucleus, Sol) were specifically activated by isoflurane, with the remaining 11 were activated by both two anesthetics. Moreover, within the 17 brain regions, ADP, SubCV (subcoeruleus nucleus, ventral part), PCRtA (parvicellular reticular nucleus, alpba part) and ION were newly identified that activated by propofol or isoflurane, respectively. By using Targeted Recombination in Active Populations (TRAP) technique, we further showed that propofol and isoflurane largely activate the same group of neurons in supraoptic nucleus (SON), but activate different groups of neurons in central amygdala (CeA). Our results reveals the neural ensembles activated by injectable and inhaled anesthetics, and provides detailed anatomical references for future studies on general anesthesia.
全身麻醉已广泛应用于外科手术。丙泊酚和异氟烷分别是最常用的注射麻醉剂和吸入麻醉剂。丙泊酚和异氟烷引起的各种不良反应与麻醉依赖的大脑活动变化高度相关。在这项研究中，我们旨在描绘注射麻醉剂与吸入麻醉剂在全脑范围内的神经元活动图谱，以了解这两种主要麻醉剂引起的不同生理效应的神经基础。通过对接受丙泊酚或异氟烷麻醉的整个小鼠大脑进行详细扫描，我们总共识别出17个皮质下区域，其中3个区域（前背侧视前核，ADP；外侧缰核，LHb；下橄榄核，ION）被丙泊酚特异性激活，3个区域（外侧隔核腹侧部，LSV；外侧隔核中间部，LSI；孤束核，Sol）被异氟烷特异性激活，其余11个区域被两种麻醉剂共同激活。此外，在这17个脑区中，ADP、SubCV（蓝斑下核腹侧部）、PCRtA（小细胞网状核α部）和ION是新发现的分别被丙泊酚或异氟烷激活的区域。通过使用活性群体靶向重组（TRAP）技术，我们进一步表明，丙泊酚和异氟烷在很大程度上激活了视上核（SON）中的同一组神经元，但在中央杏仁核（CeA）中激活了不同组的神经元。我们的研究结果揭示了注射麻醉剂和吸入麻醉剂激活的神经群体，并为未来全身麻醉的研究提供了详细的解剖学参考。
