Kelton J G, Denomme G, Lucarelli A, Garvey J, Powers P, Carter C
Am J Hematol. 1985 Jan;18(1):1-5. doi: 10.1002/ajh.2830180102.
Platelet-associated IgG (PAIgG) can be measured on intact platelets or following platelet lysis. Measurement of PAIgG following platelet lysis may provide different or additional information compared to PAIgG measured on intact platelets. The PAIgG of lysed platelets represents "total" PAIgG, ie, IgG on the surface of platelets plus any IgG that was inside the platelet. To investigate the clinical relevance of the two types of PAIgG assay we performed a prospective study on washed platelets collected from 47 patients with idiopathic thrombocytopenic purpura (ITP). The PAIgG was measured on intact and lysed platelets using an immunoradiometric assay. Platelet-associated IgG was 2-3 times higher when measured on lysed platelets from healthy controls or patients with ITP compared to PAIgG measured on the same intact platelets. The higher level of PAIgG observed following platelet lysis was not due to the reactions not achieving equilibrium. Using lysed platelets, PAIgG was elevated on 29 of 47 samples from different ITP patients and elevated in 31 samples when measured on intact platelets. The PAIgG is invariably higher when measured following platelet lysis compared measurements made on intact platelets. Neither technique offers a diagnostic advantage over the other.
血小板相关IgG（PAIgG）可以在完整血小板上或血小板裂解后进行测量。与在完整血小板上测量的PAIgG相比，血小板裂解后测量PAIgG可能会提供不同或额外的信息。裂解血小板的PAIgG代表“总”PAIgG，即血小板表面的IgG加上血小板内部的任何IgG。为了研究这两种PAIgG检测方法的临床相关性，我们对从47例特发性血小板减少性紫癜（ITP）患者采集的洗涤血小板进行了一项前瞻性研究。使用免疫放射分析在完整和裂解的血小板上测量PAIgG。与在相同完整血小板上测量的PAIgG相比，在健康对照或ITP患者的裂解血小板上测量时，血小板相关IgG高2至3倍。血小板裂解后观察到的较高水平的PAIgG并非由于反应未达到平衡。使用裂解血小板时，47例不同ITP患者的样本中有29例PAIgG升高，在完整血小板上测量时31个样本中PAIgG升高。与在完整血小板上进行的测量相比，在血小板裂解后测量时，PAIgG总是更高。两种技术都没有比另一种技术具有诊断优势。
