Kim Seong Hee, Kahng Dong Young, Kim Kyung Soo, Min Hyun Jin
Department of Otorhinolaryngology-Head and Neck Surgery, Chung-Ang University Hospital, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
J Rhinol. 2023 Nov;30(3):135-138. doi: 10.18787/jr.2023.00042. Epub 2023 Nov 27.
To date, no studies have been conducted on the interaction between extracellular heat shock protein 70 (Hsp70) and C-X-C chemokine receptor type 4 (CXCR4) in the upper airway. We aimed to evaluate the relationship between extracellular Hsp70 and CXCR4 and their role in the primary human nasal epithelium.
We cultured primary human nasal epithelial (HNE) cells in an air-liquid interface. Macrogen performed single-cell quantitative polymerase chain reaction and sequencing. We conducted western blot analysis for the CXCR4 and mitogen-activated protein kinase (MAPK) pathways.
Extracellular Hsp70 treatment significantly increased the genetic expression and protein levels of CXCR4 in primary HNE cells. Phospho-ERK expression was increased by cotreatment with Hsp70 and CXCL12, but inhibited by pretreatment with AMD3100, a CXCR4 inhibitor. Pretreatment with an anti-Hsp70 antibody reduced phospho-ERK expression upregulation induced by cotreatment with Hsp70 and CXCL12.
Extracellular Hsp70 participates in the activation of the CXCR4-dependent downstream signaling pathway in HNE cells. Further studies should evaluate the extracellular Hsp70-CXCL12/CXCR4 axis and the role of its components in the development of inflammatory diseases.
迄今为止，尚未有关于上呼吸道中细胞外热休克蛋白70（Hsp70）与C-X-C趋化因子受体4（CXCR4）之间相互作用的研究。我们旨在评估细胞外Hsp70与CXCR4之间的关系及其在原代人鼻上皮中的作用。
我们在气液界面培养原代人鼻上皮（HNE）细胞。Macrogen进行单细胞定量聚合酶链反应和测序。我们对CXCR4和丝裂原活化蛋白激酶（MAPK）通路进行了蛋白质印迹分析。
细胞外Hsp70处理显著增加了原代HNE细胞中CXCR4的基因表达和蛋白水平。Hsp70与CXCL12共同处理可增加磷酸化ERK的表达，但CXCR4抑制剂AMD3100预处理可抑制其表达。抗Hsp70抗体预处理可降低Hsp70与CXCL12共同处理诱导的磷酸化ERK表达上调。
细胞外Hsp70参与HNE细胞中CXCR4依赖性下游信号通路的激活。进一步的研究应评估细胞外Hsp70-CXCL12/CXCR4轴及其成分在炎症性疾病发生发展中的作用。
