Wang Yandan, Wei Wei, Wang Yongjin, Liu Xiaojing, Jin Qingzhe, Wang Xingguo
State Key Lab of Food Science and Resources, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Life Sciences, Anhui Normal University, Wuhu 241000, China; Jiahe Food Industry Co., Ltd, Suzhou 215000, China.
State Key Lab of Food Science and Resources, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Food Chem. 2025 Mar 15;468:142410. doi: 10.1016/j.foodchem.2024.142410. Epub 2024 Dec 9.
Medium- and long-chain triacylglycerols (MLCTs) have been widely used in the field of pharmaceutical nutrition. However, the application of MLCT as enteral nutrition remains underexplored. Our previous in vitro digestion experiment revealed that MLCTs can improve the bioaccessibility of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in comparison with physically mixed oils (MOs). In this study, we showed that MLCTs promote enhance triglyceride synthesis and secretion in Caco-2 cells, as evidenced by significant upregulation of RNA transcription levels of FATP4, L-FABP, and PPARα (p < 0.05) compared with MOs. Animal experiment results show that MLCTs can improve the energy metabolism and liver function, decrease NF-κB p65 and inflammatory cytokines, such as TNF-α, IL-6, and IL-8 of rats undergoing chemotherapy postoperatively. This was related to the elevated bioavailability and metabolic efficiency of functional fatty acids in MLCTs. Besides, RT-qPCR, western blot, and immunohistochemical analyses confirme that MLCTs downregulate the expression of proinflammatory factors by suppressing the liver NF-κB signal transduction pathway, thereby improving the nutritional status of rats.
中长链甘油三酯（MLCTs）已在医药营养领域得到广泛应用。然而，MLCT作为肠内营养的应用仍有待深入探索。我们之前的体外消化实验表明，与物理混合油（MOs）相比，MLCTs可提高二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）的生物可及性。在本研究中，我们发现MLCTs可促进Caco-2细胞中甘油三酯的合成与分泌，与MOs相比，FATP4、L-FABP和PPARα的RNA转录水平显著上调（p < 0.05）即为证明。动物实验结果表明，MLCTs可改善术后化疗大鼠的能量代谢和肝功能，降低NF-κB p65以及炎性细胞因子如TNF-α、IL-6和IL-8的水平。这与MLCTs中功能性脂肪酸的生物利用度和代谢效率提高有关。此外，RT-qPCR、蛋白质印迹和免疫组织化学分析证实，MLCTs通过抑制肝脏NF-κB信号转导途径下调促炎因子的表达，从而改善大鼠的营养状况。
