Association of M13 I-forms and spheroids with lipid vesicles.
作者信息
Manning M, Griffith J
出版信息
Arch Biochem Biophys. 1985 Jan;236(1):297-303. doi: 10.1016/0003-9861(85)90629-0.
PMID:3966795
Abstract
Electron microscopy and density gradient centrifugation were used to demonstrate that the coat protein of M13 I-forms and spheroids, but not of filaments, can form some type of association with lipid vesicles in vitro. The association was detected only when the phage particles were incubated with dilauroylphosphatidylcholine (DLPC) or dimyristoylphosphatidylcholine (DMPC) small unilamellar vesicles (SUV) above the phase transition temperature of the lipid. Under these conditions the I-form coat protein was resistant to proteolytic digestion, and the viral DNA was also associated with the vesicles.
摘要
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