基于沉降速度参数的大分子形状函数。

A macromolecular shape function based on sedimentation velocity parameters.

作者信息

Nichol L W, Owen E A, Winzor D J

出版信息

Arch Biochem Biophys. 1985 Jan;236(1):338-41. doi: 10.1016/0003-9861(85)90634-4.

Abstract

A volume-independent shape function, experimentally determinable from sedimentation velocity experiments, was formulated explicitly in terms of the axial ratio of a macromolecule considered as an ellipsoid of revolution. Use of this function offers a simple first approach to the elucidation of macromolecular geometry as illustrated by calculations for ovalbumin, bovine serum albumin, and myosin.

摘要

一个与体积无关的形状函数可通过沉降速度实验由实验确定，它根据被视为旋转椭球体的大分子的轴比明确地构建。使用这个函数为阐明大分子几何形状提供了一种简单的初步方法，如对卵清蛋白、牛血清白蛋白和肌球蛋白的计算所示。