Gao Ya, Huo Haoping, Zhang Renyuan, Gao Haonan, Liu Yancheng, Wang Yingbo
School of Chemistry and Chemical Engineering, Xinjiang Normal University, Urumqi 830054, China.
State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Guangxi Normal University, Guilin 541004, China.
ACS Appl Mater Interfaces. 2024 Dec 25;16(51):70339-70351. doi: 10.1021/acsami.4c17271. Epub 2024 Dec 12.
The stratum corneum of the skin functions as a barrier, obstructing drug absorption and complicating the treatment of skin infections caused by pathogens such as bacteria, fungi, and viruses through topical methods. In this research, a microneedle patch was developed, which consists of gelatin-sucrose (SG) that encapsulates polydopamine-copper nanoparticles (PDA@Cu) at the tip for antibacterial purposes. Poly(ethylene glycol) diacrylate (PEGDA) served as the primary substrate for the microneedle shaft (PDA@Cu-SG/PEGDA). This patch was engineered to enhance skin permeability and facilitate the efficient delivery of drugs through the rapid dissolution of the tips. Coordinate bonds can be formed between PDA and Cu ions, resulting in the immobilization of Cu particles on the PDA surface, which aids in the green synthesis of PDA@Cu photothermal antibacterial particles. The SG polymer, which contained PDA@Cu, was employed to fill the tip cavities through a series of centrifugation steps. Subsequently, UV-induced polymerization was carried out to produce PEGDA microneedles with hydrophilic tips (PDA@Cu-SG/PEGDA). Furthermore, the physicochemical characteristics of the polymer microneedles, including morphology, composition, and mechanical strength, were thoroughly characterized. These microneedles with hydrophilic tips demonstrated sufficient mechanical strength. Owing to the ability of PDA@Cu to disrupt bacterial membranes, the microneedle patch demonstrated excellent in vitro antibacterial efficacy. We validated the antibacterial activity of the microneedle patch against and by conducting in vitro bacterial plate coating assays. The results of the assays indicated that this minimally invasive strategy achieved broad-spectrum antibacterial effects; thus, this may be a suitable approach for managing polymicrobial skin infections during clinical trials.
皮肤的角质层起到屏障作用，阻碍药物吸收，使得通过局部用药治疗由细菌、真菌和病毒等病原体引起的皮肤感染变得复杂。在本研究中，开发了一种微针贴片，其由明胶 - 蔗糖（SG）组成，在尖端封装了聚多巴胺 - 铜纳米颗粒（PDA@Cu）用于抗菌目的。聚乙二醇二丙烯酸酯（PEGDA）作为微针杆（PDA@Cu - SG/PEGDA）的主要基材。该贴片经过设计，可通过尖端的快速溶解来增强皮肤渗透性并促进药物的有效递送。PDA和铜离子之间可形成配位键，导致铜颗粒固定在PDA表面，这有助于PDA@Cu光热抗菌颗粒的绿色合成。含有PDA@Cu的SG聚合物通过一系列离心步骤用于填充尖端腔。随后，进行紫外线诱导聚合以制备具有亲水性尖端的PEGDA微针（PDA@Cu - SG/PEGDA）。此外，对聚合物微针的物理化学特性，包括形态、组成和机械强度，进行了全面表征。这些具有亲水性尖端的微针表现出足够的机械强度。由于PDA@Cu破坏细菌膜的能力，微针贴片在体外表现出优异的抗菌效果。我们通过进行体外细菌平板涂布试验验证了微针贴片对[具体细菌名称1]和[具体细菌名称2]的抗菌活性。试验结果表明，这种微创策略实现了广谱抗菌效果；因此，这可能是临床试验中管理多种微生物皮肤感染的合适方法。
