Shin Jung-Woo, Shin Yoon-Jung, Lee Dong-Yun, Kim Dong-Hyun
Neurobiota Research Center, College of Pharmacy, Kyung Hee University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea.
Lett Appl Microbiol. 2024 Dec 2;77(12). doi: 10.1093/lambio/ovae128.
Helicobacter pylori (HP) causes gastritis and peptic ulcer. Therefore, we examined whether probiotics Lactococcus lactis P135 and Bifidobacterium longum P142, which inhibited HP growth by 37.9% and 35.3%, respectively, and HP-induced IL-8 expression in KATO III cells by 68.6% and 63.1%, respectively, compared to those of normal controls, could mitigate HP-induced gastritis and psychiatric disorder in mice. Oral administration of P135 and/or P142 alleviated HP- or aspirin-induced gastritis, colitis, neuroinflammation, and depression/cognitive impairment-like behavior. They also suppressed HP infection, neutrophil infiltration, and NF-κB activation in the stomach and TNF-α expression and NF-κB activation in the colon and hippocampus. of P135 and/or P142 alleviated HP- or aspirin-induced gut dysbiosis: they decreased Lachnospiracease, Helicobacteriaceae, and Akkermansiaceae populations and increased Bacteroidaceae and Muribaculaceae populations. These findings suggest that HP growth/inflammation-inhibitory P135 and/or P142 may alleviate gut inflammation (gastritis and colitis) and neuroinflammation through the suppression of neutrophil infiltration, NF-κB activation, and HP growth, thereby leading to the attenuation of systemic inflammation and psychiatric disorder.
幽门螺杆菌（HP）可引发胃炎和消化性溃疡。因此，我们研究了益生菌乳酸乳球菌P135和长双歧杆菌P142是否能够减轻小鼠因HP感染所致的胃炎和精神障碍。相较于正常对照组，这两种益生菌分别可抑制HP生长37.9%和35.3%，并分别抑制HP诱导的KATO III细胞中白细胞介素-8表达68.6%和63.1%。口服P135和/或P142可缓解HP或阿司匹林诱导的胃炎、结肠炎、神经炎症以及类似抑郁/认知障碍的行为。它们还可抑制胃中的HP感染、中性粒细胞浸润和核因子-κB（NF-κB）激活，以及结肠和海马体中的肿瘤坏死因子-α（TNF-α）表达和NF-κB激活。P135和/或P142可缓解HP或阿司匹林诱导的肠道菌群失调：它们减少了毛螺菌科、幽门螺杆菌科和阿克曼氏菌科的菌群数量，并增加了拟杆菌科和Muribaculaceae科的菌群数量。这些研究结果表明，具有抑制HP生长/炎症作用的P135和/或P142可能通过抑制中性粒细胞浸润、NF-κB激活和HP生长来减轻肠道炎症（胃炎和结肠炎）和神经炎症，从而减轻全身炎症和精神障碍。
