Katyal Moniya, Singh Rakshanda, Mahajan Ritu, Sharma Anurekha, Gupta Ranjan, Aggarwal Neeraj K, Yadav Anita
Department of Biotechnology, Kurukshetra University, Kurukshetra, Haryana, India.
Department of Electronic Science, Kurukshetra University, Kurukshetra, Haryana, India.
Biotechnol Appl Biochem. 2024 Dec 12. doi: 10.1002/bab.2706.
Nanocellulose is the renewable biopolymer produced in nature by different bacteria. The widespread use of nanocellulose in industrial processes increases the demand for this valuable biomaterial. To overcome the high cost of producing nanocellulose using the Hestrin-Schramm medium, alternative agricultural waste has been studied as a potential low-cost supply. This study investigated the optimization and physicochemical characterization of cellulose membrane obtained, utilizing a low-cost substrate--papaya peel-based medium, with Novacetimonas hansenii BMK-3.The maximum yield of nanocellulose was found at an inoculum age 24 h, inoculum size 10% (v/v), incubation time 15 days, pH 3.5, media:flask volume ratio 1:2.5, and temperature 30°C. Cellulose yield produced using the papaya peel-based medium was nearly four times more than using the Hestrin-Schramm medium. The structural and physical properties of cellulose were characterized using field emission scanning electron microscopy, Fourier-transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, and derivative of thermogravimetric analysis. Cellulose produced using papaya peel-based medium had similar properties to cellulose produced in the Hestrin-Schramm medium. The results suggested papaya peels as a cost-effective substrate for cellulose production with enhanced yield. This study reports an eco-friendly approach for the management of papaya peels waste disposal and production of value-added product. This is the first report mentioning the valorization of papaya fruit peel waste for the production of cellulose.
纳米纤维素是自然界中由不同细菌产生的可再生生物聚合物。纳米纤维素在工业过程中的广泛应用增加了对这种珍贵生物材料的需求。为了克服使用赫斯特林-施拉姆培养基生产纳米纤维素的高成本问题，人们研究了替代农业废弃物作为潜在的低成本供应源。本研究利用低成本底物——木瓜皮基培养基，与汉氏新醋杆菌BMK-3一起，对所得纤维素膜进行了优化和理化特性表征。发现纳米纤维素的最大产量出现在接种龄24小时、接种量10%（v/v）、培养时间15天、pH值3.5、培养基与烧瓶体积比1:2.5以及温度30°C的条件下。使用木瓜皮基培养基生产的纤维素产量几乎是使用赫斯特林-施拉姆培养基的四倍。使用场发射扫描电子显微镜、傅里叶变换红外光谱、X射线衍射、热重分析和热重分析导数对纤维素的结构和物理性质进行了表征。使用木瓜皮基培养基生产的纤维素与在赫斯特林-施拉姆培养基中生产的纤维素具有相似的性质。结果表明，木瓜皮是一种具有成本效益的纤维素生产底物，产量有所提高。本研究报告了一种生态友好的方法，用于处理木瓜皮废弃物并生产增值产品。这是第一份提及利用木瓜果皮废弃物生产纤维素的报告。
