Zhang Weilin, Chen Siyuan, Huang Shengbang, Li Zhencong, Wang Zhongwei, Dai Zhiwen, Liang Jinguo, Rong Hongrui, Ouyang Qianqian, Guo Weixiong, Wei Yen, Wei Jinsong
Department of Spinal Degeneration and Deformity Surgery, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, People's Republic of China.
The Marine Biomedical Research Institute, Guangdong Medical University, Zhanjiang 524023, People's Republic of China.
Biofabrication. 2024 Dec 13;17(1). doi: 10.1088/1758-5090/ad998f.
Restoration of disc height and biomechanical function is essential for intervertebral disc degeneration (IDD) treatment. Removing abnormal nucleus pulposus (NP) tissue is an important step to facilitate bony fusion during the healing process. We analyzed publicly available single-cell transcriptome data for human normal and degenerative NP to identify genes associated with NP degeneration. A novel poly(glycolide-co-caprolactone)@polylactide (PLA)-b-aniline pentamer (AP)-b-PLA/chitosan--polylysine (PGCL@1PAP/10CSPL) scaffold with good biocompatibility and electroactivity was designed and fabricated as an implant for IDD treatment using 3D printing technology. The PGCL@1PAP/10CSPL scaffold exhibited superior hydrophilicity, mechanical properties, cytocompatibility, and antibacterial activity compared to PGCL. Fibronectin 1 (FN1), identified from single-cell transcriptome analysis, was loaded into the PGCL@1PAP/10CSPL scaffold to accelerate the abnormal NP degeneration.andexperiments indicated that the PGCL@1PAP/10CSPL-FN1 scaffold enhanced osteogenic differentiation, promoted angiogenesis, and facilitated the removal of damaged disc tissue. This study introduces a novel implant system with desirable mechanical strength and unique bone-promoting and vascularizing properties for lumbar interbody fusion in IDD treatment.
恢复椎间盘高度和生物力学功能对于椎间盘退变（IDD）治疗至关重要。去除异常的髓核（NP）组织是在愈合过程中促进骨融合的重要步骤。我们分析了公开可用的人类正常和退变NP的单细胞转录组数据，以鉴定与NP退变相关的基因。设计并制造了一种具有良好生物相容性和电活性的新型聚（乙交酯-共-己内酯）@聚丙交酯（PLA）-b-苯胺五聚体（AP）-b-PLA/壳聚糖-聚赖氨酸（PGCL@1PAP/10CSPL）支架，作为使用3D打印技术治疗IDD的植入物。与PGCL相比，PGCL@1PAP/10CSPL支架表现出优异的亲水性、机械性能、细胞相容性和抗菌活性。从单细胞转录组分析中鉴定出的纤连蛋白1（FN1）被加载到PGCL@1PAP/10CSPL支架中，以加速异常NP退变。实验表明，PGCL@1PAP/10CSPL-FN1支架增强了成骨分化，促进了血管生成，并有助于去除受损的椎间盘组织。本研究介绍了一种新型植入系统，具有理想的机械强度以及独特的促进骨生长和血管化特性，用于IDD治疗中的腰椎椎间融合。
