叶蜂总科和瘿蜂总科（膜翅目）中核糖体DNA簇的数量和位置：最新进展
Number and location of rDNA clusters in the superfamilies Tenthredinoidea and Cynipoidea (Hymenoptera): an update.
作者信息
Gokhman Vladimir E, Kuznetsova Valentina G, Anokhin Boris A
机构信息
Russian Entomological Society, Moscow, Russia Russian Entomological Society Moscow Russia.
Zoological Institute, Russian Academy of Sciences, St. Petersburg, Russia Zoological Institute, Russian Academy of Sciences St. Petersburg Russia.
出版信息
Comp Cytogenet. 2024 Dec 4;18:239-246. doi: 10.3897/compcytogen.18.142301. eCollection 2024.
To identify nucleolus organizing regions (NORs), fluorescence hybridization (FISH) with 18S rDNA probe was performed on chromosomes of Linnaeus, 1758 (Tenthredinidae), (Linnaeus, 1767) (Argidae) (n = 10 in both) and (Bouché, 1834) (Cynipidae) (2n = 20). In all these species, a single pericentromeric rDNA cluster per haploid karyotype was detected. This number of NORs is confirmed as ancestral for the order Hymenoptera.
为了鉴定核仁组织区（NORs），使用18S rDNA探针进行荧光原位杂交（FISH），对1758年林奈命名的（叶蜂科）、1767年林奈命名的（三节叶蜂科）（两者n均为10）以及1834年布歇命名的（瘿蜂科）（2n = 20）的染色体进行检测。在所有这些物种中，每个单倍体核型检测到一个着丝粒周围rDNA簇。NORs的这个数量被确认为膜翅目昆虫的祖先特征。
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