Nieto C S, Garcia P B, Gomis J E, Garcia M J
Arch Otolaryngol. 1985 Jan;111(1):43-6. doi: 10.1001/archotol.1985.00800030077010.
Ninety-four myocutaneous island flaps of the pectoralis major were studied in the rabbit using sulfan blue (Disulphine-blue). In 13 cases, the dye was injected intra-arterially in the acromiothoracic artery before the flap was lifted, and 47 more were injected after the lifting of the flap. In 34 further cases, sulfan blue was injected intravenously once the flap had been lifted. In another 16 flaps, vascularization was studied by means of "diaphanization" (ie, making the tissue transparent or diaphenous in nature). In all groups, the surviving length of flap was observed to be greater than the stained length. Intra-arterial administration of sulfan blue was associated with reduced flap survival. Viable flap length was not related to the anatomy of the vascular base.
使用磺胺蓝（二磺蓝）对94个兔胸大肌肌皮岛状皮瓣进行了研究。13例在掀起皮瓣前经肩胸动脉动脉内注射染料，另外47例在掀起皮瓣后注射。在另外34例中，皮瓣掀起后经静脉注射磺胺蓝。另有16个皮瓣通过“透明化”（即使组织在本质上变得透明）研究血管化情况。在所有组中，观察到皮瓣的存活长度大于染色长度。动脉内给予磺胺蓝与皮瓣存活率降低有关。存活皮瓣长度与血管蒂解剖结构无关。
