Chisembe Pilirani, Suzuki Masato, Dao Duc Trung, Njunga Gilson, Nkhoma Joseph, Mthilakuwili Lecollins, Kinoshita-Daitoku Ryo, Kuroda Eisuke, Kimura Kouji, Shibayama Keigo
Department of Bacteriology, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa, Nagoya, Aichi 466-8850, Japan.
Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, 4-2-1 Aobacho, Higashimurayama, Tokyo 189-0002, Japan.
JAC Antimicrob Resist. 2024 Dec 12;6(6):dlae200. doi: 10.1093/jacamr/dlae200. eCollection 2024 Dec.
Antimicrobial resistance is a global health challenge with profound implications across sectors. Livestock, a significant field at the One Health interface, lacks sufficient information, particularly in low-resource settings such as Malawi.
We determined the antimicrobial resistance rates of isolated from broiler chickens in Malawi and explored the relationship between resistance genes across sectors using genomic analysis.
In 2023, we isolated 115 strains from 116 faecal and caecal samples from broiler chickens across Malawi. Antimicrobial susceptibility tests were performed using agar dilution method according to the Clinical Laboratory Standard Institute guidelines. Whole-genome sequencing was performed using Illumina sequencing.
Notably, 50 isolates (44%) were resistant to cefotaxime. We detected ESBL genes ( , , , , , , and ) in 48 cefotaxime-resistant isolates, which exhibited higher resistance rates to levofloxacin than non-ESBL-encoding isolates (29/48; 60% versus 20/67; 30%). All isolates were susceptible to colistin and carbapenems. High resistance rates were observed for tetracycline and co-trimoxazole commonly used in broiler chickens (90% and 70%, respectively). Sequence type 206 and phylogroup A were predominant (14% and 65%, respectively). In the genetic context of genes, whole-genome alignment of the ESBL-producing isolates with reference plasmids from of various origins indicated significant similarity.
Antimicrobial resistance is highly prevalent among from broiler chickens in Malawi. Genomic analysis suggests potential transmission pathways for ESBL genes across sectors, necessitating further studies from One Health perspective.
抗菌药物耐药性是一项全球性的健康挑战，对各个领域都有深远影响。畜牧业作为“同一健康”理念中一个重要领域，缺乏足够的信息，尤其是在马拉维等资源匮乏地区。
我们测定了从马拉维肉鸡中分离出的[具体细菌名称未给出]的抗菌药物耐药率，并利用基因组分析探索不同领域耐药基因之间的关系。
2023年，我们从马拉维各地的肉鸡的116份粪便和盲肠样本中分离出115株[具体细菌名称未给出]菌株。根据临床实验室标准协会指南，采用琼脂稀释法进行抗菌药物敏感性试验。使用Illumina测序进行全基因组测序。
值得注意的是，50株分离株（44%）对头孢噻肟耐药。我们在48株对头孢噻肟耐药的分离株中检测到ESBL[具体基因名称未给出]基因（[列出具体基因名称]），这些分离株对左氧氟沙星的耐药率高于非ESBL编码分离株（29/48；60%对20/67；30%）。所有分离株对黏菌素和碳青霉烯类药物敏感。在肉鸡中常用的四环素和复方新诺明的耐药率很高（分别为90%和70%）。序列类型206和系统发育群A占主导地位（分别为14%和65%）。在[具体基因名称未给出]基因的遗传背景下，产ESBL分离株与来自不同来源的[具体细菌名称未给出]参考质粒的全基因组比对显示出显著相似性。
抗菌药物耐药性在马拉维肉鸡的[具体细菌名称未给出]中高度流行。基因组分析表明ESBL基因在不同领域之间可能的传播途径，有必要从“同一健康”角度进行进一步研究。
