Dinana Zayyin, Doan Yen Hai, Maharani Aussie Tahta, Fitria Anisa Lailatul, Yamani Laura Navika, Wahyuni Rury Mega, Soegijanto Soegeng, Utsumi Takako, Matsui Chieko, Deng Lin, Takemae Nobuhiro, Kageyama Tsutomu, Katayama Kazuhiko, Lusida Maria Inge, Shoji Ikuo
Laboratory of Viral Diarrhea, Indonesia-Japan Collaborative Research Center for Emerging and Re-emerging Infectious Diseases, Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia.
Research Center on Global Emerging and Re-emerging Infectious Diseases, Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia.
J Med Virol. 2024 Dec;96(12):e70106. doi: 10.1002/jmv.70106.
Inter-genogroup reassortment of Rotavirus A (RVA) strains has highlighted the spread of unusual RVA strains worldwide. We previously reported the equine-like G3 RVA as the predominant strain in Indonesia in 2015-2016. However, since July 2017, typical human genotypes G1 and G3 have replaced these strains completely. To understand how dynamic changes in RVA occur in Indonesia, we performed a detailed epidemiological study. A total of 356 stool specimens were collected from hospitalized children in Sidoarjo, Indonesia between 2018 and 2022. Whole-genome sequencing was performed for all 26 RVA-positive samples using next-generation sequencing. Twenty-four samples were determined to be the unusual RVA G9P[4], while two were G9P[6]. Detailed analysis revealed that seven G9P[4] strains had the typical DS-1-like backbone, while the other strains exhibited a double-reassortant profile (G9-N1) on the DS-1-like backbone. The Bayesian evolutionary analyses suggested that the Indonesian G9P[4] strains share a common ancestor with previously reported G9P[4] strains in the VP7 and VP4 genes. G9P[4] DS-1-like strains were identified as the predominant genotype in Indonesia in 2021 for the first time. These results suggest that the G9P[4] strains were generated from the previous G9P[4] strains that had undergone further intra-reassortments with the other circulating strains.
轮状病毒A（RVA）株的基因间重配现象凸显了不寻常RVA株在全球范围内的传播。我们之前报道过，类马G3 RVA株在2015 - 2016年是印度尼西亚的主要毒株。然而，自2017年7月以来，典型的人类基因型G1和G3已完全取代了这些毒株。为了解印度尼西亚RVA的动态变化情况，我们开展了一项详细的流行病学研究。2018年至2022年期间，从印度尼西亚泗水县住院儿童中总共收集了356份粪便标本。使用下一代测序技术对所有26份RVA阳性样本进行了全基因组测序。确定其中24份样本为不寻常的RVA G9P[4]，另外两份为G9P[6]。详细分析显示，7株G9P[4]毒株具有典型的DS - 1样主干，而其他毒株在DS - 1样主干上呈现双重配型（G9 - N1）。贝叶斯进化分析表明，印度尼西亚的G9P[4]毒株在VP7和VP4基因上与先前报道的G9P[4]毒株有共同祖先。G9P[4] DS - 1样毒株于2021年首次被确定为印度尼西亚的主要基因型。这些结果表明，G9P[4]毒株是由先前的G9P[4]毒株与其他流行毒株进一步发生基因内重配而产生的。
