Liu Zhenwei, Xian Yuting, Lan Jixian, Zhou Zhichao, Li Xiao, Zhou Rong, Chen Dehui, Tian Xingui
Department of Pediatrics, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, China.
State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, China.
mSphere. 2025 Jan 28;10(1):e0064424. doi: 10.1128/msphere.00644-24. Epub 2024 Dec 13.
The re-emerging human adenovirus (HAdV) types 3, 7, 14, and 55 of species B have caused severe or even fatal acute respiratory disease. Therefore, the development of multivalent vaccines against HAdV types 3, 7, 14, and 55 remains an important goal. In our previous study, we identified a cross-neutralizing epitope that induced broadly reactive monoclonal neutralizing antibodies against the knob proteins of HAdV types 7, 11, 14, and 55. To study the immunogenicity of HAdV species B knob proteins, we evaluated humoral immune responses to the knob proteins and . We found that the knob proteins elicited robust binding and neutralizing antibody responses after three immunizations of mice. In addition, mouse antisera raised against the knob proteins exhibited cross-neutralizing activity against original species B members. Furthermore, immunization with a mix of HAdV-3, 7, and 55 knob proteins protected Chinese tree shrews against an experimental HAdV challenge. Our results provide insight into the immunogenicity of HAdV species B knob proteins.IMPORTANCEHuman adenovirus (HAdV) species B are common pathogens causing severe pneumonia in children, and there is currently no vaccine available. Because there are many HAdV species B types, developing broad-spectrum vaccines against HAdV species B is an important research goal. Our study revealed that immunization with recombinant HAdV species B knob proteins effectively elicited cross-neutralizing antibody responses against original species B members with protective immunity. This study provides a novel insight into the immunogenicity of HAdV species B knob proteins.
B种人类腺病毒（HAdV）的3型、7型、14型和55型重新出现，已引发严重甚至致命的急性呼吸道疾病。因此，开发针对3型、7型、14型和55型HAdV的多价疫苗仍然是一个重要目标。在我们之前的研究中，我们鉴定出一个交叉中和表位，该表位可诱导针对7型、11型、14型和55型HAdV纤突蛋白产生广泛反应性的单克隆中和抗体。为研究B种HAdV纤突蛋白的免疫原性，我们评估了对纤突蛋白 和 的体液免疫反应。我们发现，在对小鼠进行三次免疫后，纤突蛋白引发了强烈的结合和中和抗体反应。此外，针对纤突蛋白产生的小鼠抗血清对原始的B种成员表现出交叉中和活性。此外，用HAdV-3、7和55型纤突蛋白混合物进行免疫可保护树鼩免受实验性HAdV攻击。我们的结果为B种HAdV纤突蛋白的免疫原性提供了见解。重要性人类腺病毒（HAdV）B种是导致儿童严重肺炎的常见病原体，目前尚无可用疫苗。由于HAdV B种有多种类型，开发针对HAdV B种的广谱疫苗是一个重要的研究目标。我们的研究表明，用重组B种HAdV纤突蛋白进行免疫可有效引发针对原始B种成员的交叉中和抗体反应，并具有保护性免疫。本研究为B种HAdV纤突蛋白的免疫原性提供了新的见解。
