LaBonte Peyton, Packer Ryan, McInnes Holly, Rotenberg Dorith, Whitfield Anna E
Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, USA.
Methods Mol Biol. 2025;2893:119-136. doi: 10.1007/978-1-0716-4338-9_10.
Tomato spotted wilt virus (TSWV) is transmitted by insect pests from the Thripidae family, including Frankliniella occidentalis, commonly known as western flower thrips. For experimental purposes, researchers have developed methods for inoculating host plants with TSWV, allowing thrips to acquire TSWV, and verifying thrips acquisition. Plants can be inoculated with TSWV either mechanically or with thrips in the lab, but in nature, the virus is transmitted by thrips. For the study of tospovirus transmission biology and vector-virus interactions, efficient methods for mechanical and thrips transmission are essential. We have optimized these protocols for TSWV and western flower thrips, but they can also be adapted to other tospovirus-vector systems.
番茄斑萎病毒（TSWV）由蓟马科害虫传播，包括西花蓟马Frankliniella occidentalis，通常被称为西花蓟马。出于实验目的，研究人员已开发出用TSWV接种寄主植物的方法，使蓟马获得TSWV，并验证蓟马是否获得病毒。在实验室中，植物可以通过机械接种或用蓟马接种TSWV，但在自然环境中，该病毒由蓟马传播。对于番茄斑萎病毒传播生物学以及载体-病毒相互作用的研究，有效的机械接种和蓟马传播方法至关重要。我们已经针对TSWV和西花蓟马优化了这些方案，但它们也可适用于其他番茄斑萎病毒-载体系统。
